Diversity and genome dynamics of marine cyanophages using metagenomic analyses

Environmental Microbiology Reports
Yingfei MaBrian Palenik

Abstract

Cyanophages are abundant in the oceanic environment and directly impact cyanobacterial distributions, physiological processes and evolution. Two samples collected from coastal Maine in July and September 2009 were enriched for Synechococcus cells using flow cytometry and examined through metagenomic sequencing. Homology-based sequence prediction indicated cyanophages, largely myoviruses, accounted for almost half the reads and provided insights into environmental infection events. T4-phage core-gene phylogenetic reconstruction revealed unique diversity among uncultured cyanophages and reference isolates resulting in identification of a new phylogenetic cluster. Genomic comparison of reference cyanophage strains S-SM2 and Syn1 with putative homologous contigs recovered from metagenomes provided evidence that gene insertion, deletion and recombination have occurred among, and are likely important for diversification of, natural populations. Identification of putative genetic exchange between cyanophage and non-cyanophage viruses, i.e. Micromonas virus and Pelagibacter phage, supports hypotheses related to a significant role for viruses in mediating transfer of genetic material between taxonomically diverse organisms with overlapp...Continue Reading

References

Jul 1, 1983·Journal of Bacteriology·J Kuzio, A M Kropinski
Dec 15, 2000·Trends in Ecology & Evolution·Z Yang, J P Bielawski
Dec 23, 2000·Nucleic Acids Research·B L MaidakJ M Tiedje
Apr 6, 2002·Bioinformatics·Christopher LeeMark F Sharlow
Apr 27, 2002·Applied and Environmental Microbiology·Feng Chen, Jingrang Lu
Apr 17, 2003·FEMS Microbiology Reviews·Nicholas H Mann
Aug 29, 2003·Nature·Matthew B SullivanSallie W Chisholm
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Feb 10, 2004·Applied and Environmental Microbiology·Ursula DorigoJean-François Humbert
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jul 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Debbie LindellSallie W Chisholm
Apr 15, 2005·PLoS Biology·Matthew B SullivanSallie W Chisholm
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jonathan FiléeH M Krisch
Oct 14, 2005·Nature·Debbie LindellSallie W Chisholm
Mar 25, 2006·Science·Maureen L ColemanSallie W Chisholm
Nov 9, 2006·PLoS Biology·Florent E AnglyForest Rohwer
Feb 10, 2007·Science·Tammi L Richardson, George A Jackson
May 8, 2007·Molecular Biology and Evolution·Ziheng Yang
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 15, 2007·Environmental Microbiology·Peter R WeigeleRoger W Hendrix
Apr 23, 2008·Current Protocols in Bioinformatics·Mark BorodovskyRyan Mills
Jul 1, 2008·Applied and Environmental Microbiology·C Chénard, C A Suttle
Aug 5, 2008·Environmental Microbiology·Matthew B SullivanSallie W Chisholm
Oct 21, 2009·Environmental Microbiology·Matthew B SullivanSallie W Chisholm

❮ Previous
Next ❯

Citations

Oct 14, 2017·The ISME Journal·Ksenia ArkhipovaLeonid A Kulakov
Nov 2, 2014·Applied and Environmental Microbiology·Sijun HuangFeng Chen
Nov 14, 2015·Standards in Genomic Sciences·Ahyoung ChoiJang-Cheon Cho
Aug 20, 2018·Extremophiles : Life Under Extreme Conditions·Olivier ZablockiMarla Trindade

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.