Diversity and Ice Nucleation Activity of Microorganisms Collected With a Small Unmanned Aircraft System (sUAS) in France and the United States

Frontiers in Microbiology
Celia Jimenez-SanchezD G Schmale

Abstract

Many microbes relevant to crops, domestic animals, and humans are transported over long distances through the atmosphere. Some of these atmospheric microbes catalyze the freezing of water at higher temperatures and facilitate the onset of precipitation. We collected microbes from the lower atmosphere in France and the United States with a small unmanned aircraft system (sUAS). 55 sampling missions were conducted at two locations in France in 2014 (an airfield in Pujaut, and the top of Puy de Dôme), and three locations in the U.S. in 2015 (a farm in Blacksburg, Virginia, and a farm and a lake in Baton Rouge, Louisiana). The sUAS was a fixed-wing electric drone equipped with a remote-operated sampling device that was opened once the aircraft reached the desired sampling altitude (40-50 meters above ground level). Samples were collected on agar media (TSA, R4A, R2A, and CA) with and without the fungicide cycloheximide. Over 4,000 bacterial-like colonies were recovered across the 55 sUAS sampling missions. A positive relationship between sampling time and temperature and concentrations of culturable bacteria was observed for sUAS flights conducted in France, but not for sUAS flights conducted in Louisiana. A droplet freezing assay ...Continue Reading

References

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Citations

Dec 4, 2020·Environment International·Shu HuangPingqing Fu
Apr 28, 2021·Environmental Microbiome·Cheolwoon Woo, Naomichi Yamamoto
Aug 25, 2021·The Science of the Total Environment·Philipp BalohHinrich Grothe

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Datasets Mentioned

BETA
MG924929
MG924921
MG860096
MH014870
MG924922
MG924923
MG860145

Methods Mentioned

BETA
PCR

Software Mentioned

DECIPHER
BankIt
PERMANOVA
sUAS

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