Diversity and Mealybug Transmissibility of Ampeloviruses in Pineapple

Plant Disease
D M SetherJ S Hu

Abstract

Mealybug wilt of pineapple (MWP) is one of the most destructive diseases of pineapple (Ananas comosus) worldwide. At least one Ampelovirus species, Pineapple mealybug wilt associated virus-2 (PMWaV-2), and mealybug feeding are involved in the etiology of MWP. A previously undescribed Ampelovirus sharing highest homology with PMWaV-1 and a putative deletion mutant sharing highest homology with PMWaV-2 were detected with reverse transcription-polymerase chain reaction (RT-PCR) assays using degenerate primers. Results were verified with additional sequence information and by immunosorbent electron microscopy. Sequence homology between the virus tentatively designated PMWaV-3, and PMWaV-1 and PMWaV-2, decreases toward the N-terminal across the HSP70 homolog, small hydrophobic protein, and RNA-dependent RNA polymerase open reading frames (ORF). Putative PMWaV-3 could not be detected with four different monoclonal antibodies specific for PMWaV-1 and PMWaV-2. The potential deletion mutant spanning the N-terminal of the HSP70 region was obtained from a pineapple accession from Zaire maintained at the USDA-ARS National Clonal Germplasm Repository in Hawaii. Putative PMWaV-3, like PMWaV-1 and PMWaV-2, is transmissible separately or in co...Continue Reading

References

Sep 1, 1996·The Journal of General Virology·M MawassiM Bar-Joseph
Nov 10, 2001·Annual Review of Phytopathology·Alexander V Karasev
Oct 12, 2002·Archives of Virology·G P MartelliUNKNOWN ICTV Study Group on closteroviruses and allied viruses
Oct 24, 2008·Phytopathology·M E HilfS M Garnsey

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Citations

Nov 15, 2019·Pest Management Science·Jiufeng WeiFang Wang
Dec 15, 2010·Virus Genes·Michael J MelzerJohn S Hu
Apr 7, 2012·Communicative & Integrative Biology·Candice A StaffordDiane E Ullman
Jun 23, 2016·Journal of Economic Entomology·Fabian Ludwig WestermannPhilip John Lester

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