Diversity and Molecular Characterization of Mosquitoes (Diptera: Culicidae) in Selected Ecological Regions in Kenya.

F1000Research
Moni MakandaJames Mutunga

Abstract

Mosquitoes play a predominant role as leading agents in the spread of vector-borne diseases and the consequent mortality in humans. Despite reports on increase of new and recurrent mosquito borne-disease outbreaks such as chikungunya, dengue fever and Rift Valley fever in Kenya, little is known about the genetic characteristics and diversity of the vector species that have been incriminated in transmission of disease pathogens. In this study,  mosquito species were collected from Kisumu city, Kilifi town and Nairobi city and we determined their genetic diversity and phylogenetic relationships. PCR was used to amplify the partial cytochrome oxidase subunit 1 (CO1) gene of mosquito samples. Molecular-genetic and phylogenetic analysis of the partial cytochrome oxidase subunit 1 (CO1) gene were employed to identify their relationship with known mosquito species. Fourteen (14) haplotypes belonging to genus Aedes, nine (9) haplotypes belonging to genus Anopheles and twelve (12) haplotypes belonging to genus Culex were identified in this study. Findings from this study revealed a potentially new haplotype belonging to Anopheles genus and reported the first molecular characterization of Aedes cumminsii in Kenya. Sequence results reveal...Continue Reading

References

Jul 1, 1987·The American Journal of Tropical Medicine and Hygiene·F H CollinsV Finnerty
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·T P Monath
Apr 1, 1995·Emerging Infectious Diseases·D J Gubler, G G Clark
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E WoolhouseR M Anderson
Apr 11, 2000·Emerging Infectious Diseases·P Martens, L Hall
Oct 19, 2000·The American Journal of Tropical Medicine and Hygiene·M J TurellJ Oliver
Jun 27, 2001·Cadernos de saúde pública·A AnyambaC J Tucker
Mar 24, 2006·Tropical Medicine & International Health : TM & IH·V ThenmozhiB K Tyagi
Jul 25, 2006·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Ephantus J MuturiRobert Novak
Jan 26, 2007·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Joseph MwangangiRobert Novak
Feb 23, 2007·The New England Journal of Medicine·Rémi N CharrelDidier Raoult
Dec 9, 2008·The Medical Clinics of North America·Annelies Wilder-Smith, Duane J Gubler
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Feb 4, 2010·Acta Tropica·Janet T MidegaGuiyun Yan
Nov 13, 2010·Nucleic Acids Research·Dennis A BensonEric W Sayers
Aug 26, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·J R LoaizaJ E Conn
Dec 30, 2011·PLoS Neglected Tropical Diseases·Roberto BarreraAndrew J MacKay
Jul 12, 2012·Systematic Biology·Daniel H Huson, Celine Scornavacca
Oct 16, 2012·Science·Amy WesolowskiCaroline O Buckee
May 25, 2013·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Joel LutomiahRosemary Sang
Jun 25, 2013·Acta Tropica·Eliningaya J KwekaGuiyun Yan
Jan 30, 2014·Memórias do Instituto Oswaldo Cruz·Jeffrey R Powell, Walter J Tabachnick
Jul 17, 2014·Vector Borne and Zoonotic Diseases·Stephen Higgs
Jul 22, 2014·International Journal of Environmental Research and Public Health·Jan C Semenza
Sep 12, 2015·Parasitology Research·Kadarkarai MuruganGiovanni Benelli
Nov 26, 2015·Trends in Parasitology·Moritz U G KraemerNick Golding
Mar 1, 2016·Reviews in Medical Virology·Marycelin BabaDaniel K Masiga
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Apr 14, 2016·Malaria Journal·Vera Margarete ScarpassaJosé Ferreira Saraiva
Feb 6, 2017·The Lancet Infectious Diseases·Andrea GroutElizabeth M Speakman

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Datasets Mentioned

BETA
MK300237
MK300225
MK300228
MK300229
HQ688297.1
KX420485.1
KX420429.1
KU380400.1
KX420485
MK300218

Methods Mentioned

BETA
PCR

Software Mentioned

Blastn
Chromas Lite
Jalview
Dendroscope
Software for Molecular Evolutionary Genetics ( MEGA7 )
Seaview
DnaSP
ClustalX

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