Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B" subunits of protein phosphatase 2A.

BMC Genomics
Karen ZwaenepoelVeerle Janssens

Abstract

Protein phosphatase 2A (PP2A) is a serine/threonine-specific phosphatase displaying vital functions in growth and development through its role in various signalling pathways. PP2A holoenzymes comprise a core dimer composed of a catalytic C and a structural A subunit, which can associate with a variable B-type subunit. The importance of the B-type subunits for PP2A regulation cannot be overestimated as they determine holoenzyme localisation, activity and substrate specificity. Three B-type subunit families have been identified: PR55/B, PR61/B' and PR72/B", of which the latter is currently the least characterised. We deduced the sequences and genomic organisation of the different murine PR72/B" isoforms: three genes encode nine isoforms, five of which are abundantly expressed and give rise to genuine PP2A subunits. Thereby, one novel subunit was identified. Using Northern blotting, we examined the tissue-specific and developmental expression of these subunits. All subunits are highly expressed in heart, suggesting an important cardiac function. Immunohistochemical analysis revealed a striated expression pattern of PR72 and PR130 in heart and skeletal muscle, but not in bladder smooth muscle. The subcellular localisation and cell ...Continue Reading

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Citations

Sep 10, 2013·Molecular Pain·Yun WangXuan Zhang
Feb 4, 2012·The FEBS Journal·Victoria Kolupaeva, Veerle Janssens
Jun 27, 2009·Biochemical and Biophysical Research Communications·Veerle JanssensJozef Goris
Jan 8, 2015·Frontiers in Oncology·Dorien HaesenVeerle Janssens
Nov 16, 2016·International Journal of Molecular Sciences·Jie YangFang Zheng
Apr 24, 2021·Cardiovascular Research·Shuchi GuoDouglas G Tilley

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Datasets Mentioned

BETA
BAE21013
AAC98973
and
BAE25309
BAC31413
BAC37349
BE852268
CA984544
AV356684
BB357167

Methods Mentioned

BETA
Immunoprecipitation
immunoprecipitations
pull down
via
transfection
PCR

Software Mentioned

System [UNK]
multalin
ENSEMBLE Synteny View
NetNES
EMBOSS
Nucleotide BLAST
WinMDI
BLAST
BLASTN
ENSEMBLE Contig View

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