Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Birgitta E SundströmTorgny Stigbrand

Abstract

The cytokeratins 8, 18, and 19, expressed in many normal and malignant epithelial cells, were purified from human gastrointestinal tumors and used as immunogen for hybridoma generation. The reactivity pattern of five of the generated ten monoclonal antibodies (MAb) was characterized biochemically and immunohistochemically. All of the generated MAb were reactive with the central rod portion of the cytokeratins, as determined after partial enzymatic degradation, and displayed characteristic reactivity patterns. MAb TS 4 exhibits pan-epithelial immunohistochemical reactivity staining of all epithelial structures, including all layers of epidermis and non-keratinizing squamous epithelium and myoepithelial cells. The determinant involved is present on several different cytokeratins, i.e., nos. 1, 5, 7, 8, and 15, as determined by immunoblotting experiments from different tissues and cell lines. MAb TS 1, TS 3, and TS 7 reveal pluri-epithelial reactivity pattern immunohistochemically, similar to TS 4, but they are unreactive with whole epidermis and with superficial cell layers of non-keratinizing squamous cells. MAb TS 1 was found to be highly specific and reactive only with cytokeratin 8. Furthermore, the TS 1 MAb alone can precipi...Continue Reading

Citations

Mar 6, 2002·Cancer·Amanda JohanssonTorgny Stigbrand
May 2, 2003·European Journal of Nuclear Medicine and Molecular Imaging·David ErikssonTorgny Stigbrand
Jan 30, 2004·Oral Oncology·Ojaswini S UpasaniAvinash N Bhisey
Aug 17, 2010·Cancer Biotherapy & Radiopharmaceuticals·Rozbeh JafariBirgitta E Sundström
Dec 1, 1996·Anatomia, histologia, embryologia·M Zedda, V Farina
Jan 1, 1996·Annual Review of Psychology·E SundstromC Asmus
Sep 23, 2006·Molecular Immunology·Patrik HolmBirgitta E Sundström
Jun 28, 2005·Molecular Immunology·Ann ErlandssonBirgitta Elisabeth Sundström
Oct 15, 1990·International Journal of Cancer. Journal International Du Cancer·B Sundström, T Stigbrand
Dec 24, 1997·Cancer·T StigbrandS O Hietala
Aug 1, 1993·Cancer·P StieberA Fateh-Moghadam
Jan 1, 1993·Acta Oto-laryngologica. Supplementum·M AnnikoT Stigbrand
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