Diversity of active chemolithoautotrophic prokaryotes in the sulfidic zone of a Black Sea pelagic redoxcline as determined by rRNA-based stable isotope probing

FEMS Microbiology Ecology
Sabine GlaubitzKlaus Jürgens

Abstract

Marine pelagic redoxclines are characterized by pronounced activities of chemolithoautotrophic microorganisms. As evidenced by the high dark CO(2) fixation rates measured around the oxic-anoxic interface but also in the upper sulfidic zone, the accordant organisms participate in important biogeochemical transformations. Although Epsilonproteobacteria have been identified as an important chemoautotrophic group in these environments, detailed species-level information on the identity of actively involved prokaryotes is lacking. In the present study, active chemolithoautotrophic prokaryotic assemblages were identified in the sulfidic zone of a pelagic Black Sea redoxcline by applying rRNA-based stable isotope probing in combination with 16S rRNA gene single-strand conformation polymorphism analysis and 16S rRNA gene cloning. The results showed that a single epsilonproteobacterium, affiliated with the genus Sulfurimonas, and two different members of the gammaproteobacterial sulfur oxidizer (GSO) cluster were responsible for dark CO(2) fixation activities in the upper sulfidic layer of the Black Sea redoxcline. Phylogenetically, these organisms were closely related to microorganisms, distributed worldwide, that are thought to be key...Continue Reading

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Citations

May 31, 2011·Applied and Environmental Microbiology·Graham M PumphreyJim C Spain
Sep 29, 2011·Environmental Microbiology·Claudia Wylezich, Klaus Jürgens
Apr 27, 2011·Environmental Microbiology·Violetta La ConoMichail M Yakimov
Nov 23, 2011·Biochemical Society Transactions·Frank J Stewart
Jan 19, 2012·FEMS Microbiology Ecology·Clara A FuchsmanJames W Murray
Jun 17, 2011·FEMS Microbiology Ecology·Irene RoalkvamIda Helene Steen
Oct 6, 2019·Applied and Environmental Microbiology·Cameron M CallbeckMarcel M M Kuypers
Oct 2, 2020·Environmental Microbiology·Daan M van VlietIrene Sánchez-Andrea

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