Diversity of Ammonia Oxidation (amoA ) and Nitrogen Fixation (nifH ) Genes in Lava Caves of Terceira, Azores, Portugal

Geomicrobiology Journal
Jennifer J Marshall HathawayDiana E Northup

Abstract

Lava caves are an understudied ecosystem in the subterranean world, particularly in regard to nitrogen cycling. The diversity of ammonia oxidation (amoA) and nitrogen fixation (nifH) genes in bacterial mats collected from lava cave walls on the island of Terceira (Azores, Portugal) was investigated using denaturing gradient gel electrophoresis (DGGE). A total of 55 samples were collected from 11 lava caves that were selected with regard to surface land use. Land use types above the lava caves were categorized into pasture, forested, and sea/urban, and used to determine if land use influenced the ammonia oxidizing and nitrogen fixing bacterial communities within the lava caves. The soil and water samples from each lava cave were analyzed for total organic carbon, inorganic carbon, total nitrogen, ammonium, nitrate, phosphate and sulfate, to determine if land use influences either the nutrient content entering the lava cave or the nitrogen cycling bacteria present within the cave. Nitrosospira-like sequences dominated the ammonia-oxidizing bacteria (AOB) community, and the majority of the diversity was found in lava caves under forested land. The nitrogen fixation community was dominated by Klebsiella pneumoniae-like sequences, a...Continue Reading

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Citations

Jul 2, 2019·Emerging Microbes & Infections·Nadia Rodríguez-MedinaUlises Garza-Ramos

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Datasets Mentioned

BETA
HM461331-
HM461407
HM461408-HM461553

Methods Mentioned

BETA
PCR
454 sequencing

Software Mentioned

mothur
PAUP
Sequencher
MUSCLE

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