Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Systematic and Applied Microbiology
Tomeu ViverRamon Rosselló-Mora

Abstract

The culturable fraction of aerobic, heterotrophic and extremely halophilic microbiota retrieved from sediment and brine samples of eight sampling sites in the Mediterranean, Canary Islands and Chile was studied by means of a tandem approach combining large-scale cultivation, MALDI-TOF MS targeting whole cell biomass, and phylogenetic reconstruction based on 16S rRNA gene analysis. The approach allowed the identification of more than 4200 strains and a comparison between different sampling sites. The results indicated that the method constituted an excellent tool for the discovery of taxonomic novelty. Four new genera and nine new species could be identified within the archaeal family Halobacteriaceae, as well as one new bacterial species, and a representative of Salinibacter ruber phylotype II, a group that had been refractory to isolation for the last fifteen years. Altogether, the results indicated that in order to provide better yields for the retrieval of novel taxa from the environment, performance of non-redundant environment sampling is recommended together with the screening of large sets of strains.

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Citations

Aug 16, 2015·Systematic and Applied Microbiology·Roberto VidalRamon Rosselló-Móra
Jul 15, 2015·Systematic and Applied Microbiology·Merit Del Rocío Mora-RuizRamon Rosselló-Móra
Jul 30, 2015·Systematic and Applied Microbiology·Sara Cortés-LaraRamon Rosselló-Móra
May 23, 2015·Systematic and Applied Microbiology·Karl-Heinz SchleiferRamon Rosselló-Móra
May 21, 2016·Antonie van Leeuwenhoek·Wen-Mei XuHeng-Lin Cui
Oct 27, 2016·Archives of Microbiology·Jia-Qi XuHeng-Lin Cui
Oct 16, 2016·Environmental Research·Natalija Topić PopovićRozelindra Čož-Rakovac
Nov 5, 2016·Marine Environmental Research·Esther Rubio-PortilloAlfonso A Ramos-Esplá
Jul 5, 2016·International Journal of Systematic and Evolutionary Microbiology·Koji MoriKen-Ichiro Suzuki
Jan 9, 2020·International Journal of Systematic and Evolutionary Microbiology·Yang-Jie ZhaoHeng-Lin Cui
Nov 14, 2019·Antonie van Leeuwenhoek·Eduarda AlmeidaOlga M Lage

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