Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species.

Applied and Environmental Microbiology
Ana BeloquiPeter N Golyshin

Abstract

The guts and casts of earthworms contain microbial assemblages that process large amounts of organic polymeric substrates from plant litter and soil; however, the enzymatic potential of these microbial communities remains largely unexplored. In the present work, we retrieved carbohydrate-modifying enzymes through the activity screening of metagenomic fosmid libraries from cellulose-depleting microbial communities established with the fresh casts of two earthworm species, Aporrectodea caliginosa and Lumbricus terrestris, as inocula. Eight glycosyl hydrolases (GHs) from the A. caliginosa-derived community were multidomain endo-beta-glucanases, beta-glucosidases, beta-cellobiohydrolases, beta-galactosidase, and beta-xylosidases of known GH families. In contrast, two GHs derived from the L. terrestris microbiome had no similarity to any known GHs and represented two novel families of beta-galactosidases/alpha-arabinopyranosidases. Members of these families were annotated in public databases as conserved hypothetical proteins, with one being structurally related to isomerases/dehydratases. This study provides insight into their biochemistry, domain structures, and active-site architecture. The two communities were similar in bacteri...Continue Reading

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Citations

Apr 2, 2013·Applied and Environmental Microbiology·Mónica Martínez-MartínezManuel Ferrer
Dec 21, 2010·Applied and Environmental Microbiology·Carola Simon, Rolf Daniel
Sep 19, 2015·Critical Reviews in Biotechnology·Salvatore MontellaVincenza Faraco
Aug 16, 2014·Microbial Biotechnology·María-Eugenia GuazzaroniRichard John Ward
Apr 7, 2015·Applied Microbiology and Biotechnology·Christian NyffeneggerJørn Dalgaard Mikkelsen
Nov 19, 2013·Biotechnology Advances·Stijn LagaertGuido Volckaert
Aug 18, 2016·Research in Microbiology·Khaled F A AbdelrhmanAlessio Mengoni
Feb 25, 2015·Applied and Environmental Microbiology·Alexandre B de MenezesPeter H Thrall
May 30, 2017·Applied Microbiology and Biotechnology·Cheng-Jie DuanJia-Xun Feng
Nov 9, 2019·International Journal of Molecular Sciences·Ali RohmanNi Nyoman Tri Puspaningsih
Aug 18, 2017·Applied Biochemistry and Biotechnology·Aravind MadhavanAshok Pandey
Nov 4, 2017·Environmental Science and Pollution Research International·Berenice Ordoñez-ArévaloM Angeles Calixto-Romo
Jul 13, 2018·Microorganisms·Juan-José Escuder-RodríguezMaría-Isabel González-Siso
Nov 1, 2021·The Science of the Total Environment·Berenice Ordoñez-ArévaloKarina Guillén-Navarro

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