Abstract
To obtain evidence for selection of antigens used in genetically engineered vaccine against Helicobacter pylori (H pylori). Enzyme linked immunoabsorbent assay (ELISA) was established on the basis of recombinant protein antigens rUreB, rHpaA, rVacA, rCagA1, rNapA, rFlaA and rFlaB of H pylori to detect expression rates of the antigens in bacterial isolates as well as positive rates of the antibodies in sera from H pylori-infected patients. PCR was applied to the detection of carrying rates of the genes encoding antigens in the isolates. The outputs of rUreB, rHpaA, rVacA, rCagA1, rNapA, rFlaA and rFlaB were approximately 35%, 32%, 15%, 23%, 56%, 25% and 20% of the total bacterial proteins, respectively. One hundred and fifty-one strains of H pylori were isolated from 347 biopsy specimens of chronic gastritis, peptic ulcer or gastric adenocarcinoma, with a positive rate of 43.5%. All of the isolates expressed UreB, HpaA, FlaA and FlaB while 52.3%, 92.1% and 93.4% of the isolates expressed VacA, CagA and NapA, respectively. In the sera of 151 H pylori-infected patients, the positive rates of IgG antibodies against UreB, HpaA, VacA, CagA, NapA, FlaA and FlaB were 100%, 87.4%, 43%, 71.5%, 89.4%, 84.8% and 79.5%, respectively. Furthe...Continue Reading
References
Jun 4, 1983·Lancet·J R Warren, B Marshall
Jun 16, 1984·Lancet·B J Marshall, J R Warren
Jul 28, 1995·The Journal of Biological Chemistry·J C AthertonT L Cover
Jun 1, 1995·Infection and Immunity·D J EvansP R Kvietys
Jan 1, 1993·Scandinavian Journal of Gastroenterology. Supplement·P Sipponen, H Hyvärinen
May 20, 1996·The American Journal of Medicine·R H Hunt
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S CensiniA Covacci
Jul 1, 1997·Journal of Clinical Microbiology·Y ItoM Kuriyama
Aug 7, 1997·Nature·J F TombJ C Venter
Nov 21, 1998·Gut·P J JenksA Labigne
Jan 20, 2000·Infection and Immunity·T TanahashiJ Imanishi
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SteinA Covacci
May 3, 2000·The Journal of Experimental Medicine·B SatinF Rossi
Mar 13, 2001·Annual Review of Immunology·G Del GiudiceR Rappuoli
May 10, 2002·Wiener medizinische Wochenschrift·Brigitte Dragosics
Jun 24, 2003·Infection and Immunity·Petra VolandChristian Prinz
Jul 11, 2003·World Journal of Gastroenterology : WJG·Ya-Fei MaoShu-Ping Li
Sep 3, 2003·Helicobacter·Rui Juan Du, Bow Ho
Oct 17, 2003·World Journal of Gastroenterology : WJG·Jie YanShu-Ping Li
Nov 8, 2003·Endoscopy·S RedéenK Borch
Dec 4, 2003·Clinical and Laboratory Haematology·S M Lewis, A Osei-Bimpong
Mar 7, 2006·Journal of Clinical Microbiology·Billie VelapatiñoDouglas E Berg
Jul 18, 2008·Leukemia·S-i FujiwaraH Mano
Jul 25, 2009·Expert Review of Vaccines·Giuseppe Del GiudiceRino Rappuoli
Citations
May 30, 2013·Current Microbiology·Xueyong HuangChunhua Song
Apr 12, 2013·PloS One·Kuo-Shun ShihHua-Wen Fu
May 17, 2014·World Journal of Gastroenterology : WJG·Hua-Wen Fu
Mar 26, 2011·Helicobacter·Inês VitorianoMónica Roxo-Rosa
Aug 2, 2011·Microbial Pathogenesis·Ziba V MalekshahiWalead Ebrahimizadeh
Sep 17, 2010·International Journal of Medical Microbiology : IJMM·Edith LahnerBruno Annibale
Mar 7, 2018·Advanced Healthcare Materials·Leon Chien-Wei LinChe-Ming Jack Hu
May 27, 2017·BioImpacts : BI·Farnaz FahimiYadollah Omidi
Jan 23, 2016·World Journal of Clinical Cases·Maliheh SafaviAlireza Foroumadi
Apr 27, 2017·Current Microbiology·Haiying Gu
Jun 6, 2017·Critical Reviews in Microbiology·Nasrin MirzaeiHajieh Ghasemian Safaei
Jun 27, 2019·Journal of Cellular Biochemistry·Nidhi GuptaVijay K Verma
Aug 28, 2020·Frontiers in Microbiology·Maria Felicia SoluriClelia Peano
Dec 2, 2020·Helicobacter·Jo-Lewis Banga NdzouboukouXionglin Fan
Dec 31, 2020·Cells·Jacek BajRyszard Maciejewski