Diversity of heterotrophic nitrogen fixation genes in a marine cyanobacterial mat.

Applied and Environmental Microbiology
J P ZehrH W Paerl

Abstract

The diversity of nitrogenase genes in a marine cyanobacterial mat was investigated through amplification of a fragment of nifH, which encodes the Fe protein of the nitrogenase complex. The amplified nifH products were characterized by DNA sequencing and were compared with the sequences of nitrogenase genes from cultivated organisms. Phylogenetic analysis showed that similar organisms clustered together, with the exception that anaerobic bacteria clustered together, even though they represented firmicutes, (delta)-proteobacteria, and (gamma)-proteobacteria. Mat nifH sequences were most closely related to those of the anaerobes, with a few being most closely related to the cluster of (gamma)-proteobacteria containing Klebsiella and Azotobacter species. No cyanobacterial nifH sequences were found from the mat collected in November when Microcoleus chthonoplastes was the dominant cyanobacterium, but sequences closely related to the cyanobacterium Lyngbya lagerheimeii were found during summer, when a Lyngbya strain was dominant. The results indicate that there is a high diversity of heterotrophic nitrogen-fixing organisms in marine cyanobacterial mats.

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Citations

Apr 7, 2004·Applied and Environmental Microbiology·Steven M ShortJonathan P Zehr
Apr 29, 2006·Microbial Ecology·Javier A Izquierdo, Klaus Nüsslein
Jun 20, 2008·The ISME Journal·Ina Severin, Lucas J Stal
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Mar 7, 2014·Antonie van Leeuwenhoek·Kwang Kyu KimJung-Sook Lee
Jul 9, 2014·Applied and Environmental Microbiology·Rahul A BahulikarMichael K Udvardi
Apr 26, 2001·Environmental Microbiology·H W PaerlR P Reid
Jun 6, 2003·Applied and Environmental Microbiology·Quok-Cheong ChooNazalan Najimudin
Jan 26, 2005·FEMS Microbiology Letters·Jigjiddorj Enkh-AmgalanTatsuji Seki
Jun 23, 2005·Microbial Ecology·T F Steppe, H W Paerl
Jan 18, 2008·FEMS Microbiology Ecology·Karolina BauerBirgitta Bergman
Feb 8, 2006·Applied and Environmental Microbiology·Anthony C YannarellHans W Paerl
May 4, 2011·Geobiology·T BauersachsJ S Sinninghe Damsté
Feb 12, 2009·Geobiology·T M Quan, P G Falkowski
Jul 15, 2020·Annual Review of Microbiology·Caroline S Harwood
Jun 22, 2010·Environmental Microbiology·Victoria J Bertics, Wiebke Ziebis
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Mar 10, 2004·Applied and Environmental Microbiology·Grieg F StewardJonathan P Zehr
Jun 30, 2016·MicrobiologyOpen·Kazi Md AzimuddinKoji Hamasaki
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