Diversity of microbes associated with the marine sponge, Haliclona simulans, isolated from Irish waters and identification of polyketide synthase genes from the sponge metagenome

Environmental Microbiology
Jonathan KennedyJulian R Marchesi

Abstract

Samples of the sponge Haliclona simulans were collected from Irish waters and subjected to a culture-independent analysis to determine the microbial, polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) diversity. 16S rRNA gene libraries were prepared from total sponge, bacterial enriched sponge and seawater samples. Eight phyla from the Bacteria were detected in the sponge by phylogenetic analyses of the 16S rRNA gene libraries. The most abundant phylum in the total sponge library was the Proteobacteria (86%), with the majority of these clones being from the gamma-Proteobacteria (77%); two groups of clones were dominant and together made up 69% of the total. Both of these groups were related to other sponge-derived microbes and comprised novel genera. Within the other bacterial phyla groups of clones representing novel candidate genera within the phyla Verrucomicrobia and Lentisphaerae were also found. Selective enrichment of the bacterial component of the sponge prior to 16S rRNA gene analysis resulted in a 16S rRNA gene library dominated by a novel genus of delta-Proteobacteria, most closely related to the Bdellovibrio. The potential for the sponge microbiota to produce secondary metabolites was also analysed ...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 3, 2001·Applied and Environmental Microbiology·N S WebsterR T Hill
Jan 26, 1996·The Journal of Organic Chemistry·Anthony G. M. BarrettTerri L. Boehm
Oct 24, 2001·The Journal of Organic Chemistry·Karen L. EricksonMichael R. Boyd
Jan 5, 2002·Journal of Natural Products·R J ClarkJ N Hooper
Jul 12, 2002·Applied Microbiology and Biotechnology·P ProkschR Ebel
Aug 30, 2002·Applied and Environmental Microbiology·Ute HentschelBradley S Moore
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·C Richard Hutchinson
Jun 12, 2003·FEMS Microbiology Letters·Andrew E BerryElizabeth M H Wellington
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·F O GlöcknerR Reinhardt
Aug 20, 2003·Marine Biotechnology·R V SnyderK S Rein
Dec 26, 2003·Chembiochem : a European Journal of Chemical Biology·Jörn PielDequan Hui
Feb 12, 2004·Environmental Microbiology·Nicole S WebsterChristopher N Battershill
Apr 10, 2004·Bioinformatics·Thomas HuberPhilip Hugenholtz
Jun 9, 2004·Applied and Environmental Microbiology·Lars FieselerUte Hentschel
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Sep 4, 2004·Applied and Environmental Microbiology·Patrick D SchlossJo Handelsman
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jörn PielShigeki Matsunaga
Mar 5, 2005·Applied and Environmental Microbiology·Patrick D Schloss, Jo Handelsman
Sep 10, 2005·Nature Biotechnology·David H Sherman
Oct 7, 2005·Nature·Laila P Partida-Martinez, Christian Hertweck
Dec 15, 2005·Nucleic Acids Research·Haeyoung JeongJihyun F Kim
Jan 20, 2006·FEMS Microbiology Ecology·Ute HentschelMichael W Taylor
Mar 7, 2006·Applied and Environmental Microbiology·Tae Kyung KimJohn A Fuerst
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Sep 8, 2006·Applied and Environmental Microbiology·Kevin E AshelfordAndrew J Weightman

❮ Previous
Next ❯

Citations

Dec 4, 2009·Marine Biotechnology·Mona RadwanRussell T Hill
Jan 31, 2013·Antonie van Leeuwenhoek·Klementyna Karlińska-Batres, Gert Wörheide
Oct 22, 2009·Natural Product Reports·Sean F BradyEric W Schmidt
Apr 10, 2010·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Saket NavlakhaCarl Kingsford
Nov 9, 2010·Applied and Environmental Microbiology·Robert W PhelanTeresa M Barbosa
Jan 29, 2013·Molekuliarnaia biologiia·O V KaliuzhnaiaB V Itskovich
Jul 1, 2011·Biology Direct·Robert G Beiko
Dec 5, 2012·PLoS Neglected Tropical Diseases·Lucas DiasCarla C Romano
Jan 5, 2014·PloS One·Stephen A JacksonAlan D W Dobson
Mar 29, 2014·PloS One·Jonathan KennedyAlan D W Dobson
Feb 26, 2013·Marine Drugs·Keiichi KonokiMari Yotsu-Yamashita
Jan 1, 2010·Marine Biology·Detmer Sipkema, Harvey W Blanch
Dec 17, 2014·Marine Drugs·Susan P CrowleyAlan D W Dobson
Dec 4, 2015·International Journal of Molecular Sciences·Rafael R C CuadratAlberto M R Dávila
Apr 15, 2016·Pharmacognosy Magazine·Shaza Mohamed Al-MassaraniHoong-Kun Fun
Nov 16, 2010·Journal of Basic Microbiology·Santhi V Satheeja, Solomon R D Jebakumar
Mar 26, 2011·Journal of Applied Microbiology·J A O'HalloranA D W Dobson
Nov 29, 2014·Journal of Applied Microbiology·A HoppersN B Lopanik
Aug 14, 2012·FEMS Microbiology Ecology·Emily C GilesSusanne Schmitt
Aug 12, 2009·FEMS Microbiology Letters·Hui TangLiping Zhang
Apr 21, 2010·FEMS Microbiology Ecology·Julie B Olson, Christina A Kellogg
Nov 16, 2010·Chembiochem : a European Journal of Chemical Biology·Thomas HochmuthJörn Piel
Jul 24, 2018·Frontiers in Microbiology·Akram NajafiIraj Nabipour
Sep 8, 2018·Marine Drugs·Katja GemperleinRolf Müller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.