Diversity of Nitrogen Fixation Genes in the Symbiotic Intestinal Microflora of the Termite Reticulitermes speratus.

Applied and Environmental Microbiology
Moriya OhkumaToshiaki Kudo

Abstract

The diversity of nitrogen-fixing organisms in the symbiotic intestinal microflora of a lower termite, Reticulitermes speratus, was investigated without culturing the resident microorganisms. Fragments of the nifH gene, which encodes the dinitrogenase reductase, were directly amplified from the DNA of the mixed microbial population in the termite gut and were clonally isolated. The phylogenetic analysis of the nifH product amino acid sequences showed that there was a remarkable diversity of nitrogenase genes in the termite gut. A large number of the termite nifH sequences were most closely related to those of a firmicute, Clostridium pasteurianum, with a few being most closely related to either the (gamma) subclass of the proteobacteria or a sequence of Desulfovibrio gigas. Some of the others were distantly related to those of the bacteria and were seemingly derived from the domain Archaea. The phylogenetic positions of these nifH sequences corresponded to those of genera found during a previous determination of rRNA-based phylogeny of the termite intestinal microbial community, of which a majority consisted of new, yet-uncultivated species. The results revealed that we have little knowledge of the organisms responsible for nitr...Continue Reading

References

Feb 1, 1977·Applied and Environmental Microbiology·C J Potrikus, J A Breznak
Aug 1, 1976·Journal of General Microbiology·J R FrenchJ F Bradbury
Jan 1, 1986·Annual Review of Microbiology·G J OlsenD A Stahl
Nov 1, 1989·Journal of Molecular Evolution·P Normand, J Bousquet
Aug 31, 1973·Nature·J A BreznakH C Coppel
Jan 1, 1982·Annual Review of Microbiology·J A Breznak
Feb 1, 1994·Applied and Environmental Microbiology·E D KopczynskiD M Ward
Mar 1, 1994·Applied and Environmental Microbiology·J Ben-Porath, J P Zehr
Feb 1, 1996·Applied and Environmental Microbiology·M Ohkuma, T Kudo
Jul 1, 1995·Applied and Environmental Microbiology·J P ZehrH W Paerl
Jul 13, 1973·Science·J R Benemann
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

❮ Previous
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Citations

Aug 31, 2000·Applied and Environmental Microbiology·C R LovellC E Bagwell
Apr 25, 2001·Applied and Environmental Microbiology·F PolyL J Monrozier
Jun 6, 2003·Applied and Environmental Microbiology·Quok-Cheong ChooNazalan Najimudin
Jan 11, 2005·Applied and Environmental Microbiology·María ValdésAnn M Hirsch
Mar 5, 2015·Microbes and Environments·Jun-ichi InoueMoriya Ohkuma
Dec 15, 2015·PloS One·Alain BraumanEdouard Miambi
Apr 29, 2006·Microbial Ecology·Javier A Izquierdo, Klaus Nüsslein
May 5, 2005·FEMS Microbiology Letters·Masahiro GotoTadakatsu Yoneyama
Jun 26, 2003·Environmental Microbiology·Jonathan P ZehrGrieg F Steward
Nov 6, 2012·Applied and Environmental Microbiology·Stefanie KautzCorrie S Moreau
Oct 13, 2012·Critical Reviews in Biotechnology·Awadhesh Kumar ShuklaSuresh Kumar Dubey
Jun 30, 2016·MicrobiologyOpen·Kazi Md AzimuddinKoji Hamasaki
Nov 11, 2003·Canadian Journal of Microbiology·Birgit ReiterAngela Sessitsch
Mar 17, 1999·FEMS Microbiology Letters·M OhkumaT Kudo
Jul 9, 2014·Applied and Environmental Microbiology·Yolanda MoraJaime Mora
May 29, 2016·Applied and Environmental Microbiology·Vera TaiPatrick J Keeling
Dec 21, 2019·FEMS Microbiology Ecology·Nathalia Cavichiolli de Oliveira, Fernando Luís Cônsoli
Mar 10, 2004·Applied and Environmental Microbiology·Grieg F StewardJonathan P Zehr
Dec 27, 2016·Frontiers in Microbiology·Panagiotis SapountzisMichael Poulsen
Feb 7, 2018·Scientific Reports·Nitsan Bar-ShmuelMichal Segoli
Jan 9, 2021·Annual Review of Entomology·Michael E Scharf, Brittany F Peterson
Jul 1, 1997·Journal of Bacteriology·T HurekB Reinhold-Hurek
Jun 30, 2001·Science·T G LilburnJ A Breznak

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