Diversity of spore-forming bacteria in cattle manure, slaughterhouse waste and samples from biogas plants

Journal of Applied Microbiology
E BaggeK-E Johansson

Abstract

As biowaste intended for biogas production can contain pathogenic micro-organisms, the recommended treatment is pasteurization at 70°C for 60min. This reduces pathogens such as Salmonella spp., whereas spore-forming bacteria (Bacillus spp. and Clostridium spp.) survive. Most spore-forming bacteria are harmless, but some can cause diseases such as blackleg, botulism and anthrax. In this study, the effect of the biogas process on Bacillus spp. and Clostridium spp. was investigated. We analysed 97 faecal samples, 20 slaughterhouse waste samples and 60 samples collected at different stages in the biogas process. Bacillus spp. and Clostridium spp. were quantified and subcultured. The isolates were identified by biochemical methods and by 16S rRNA gene sequencing. Phylogenetic trees were constructed from the sequences obtained from isolates from the samples. Clostridium botulinum/Clostridium spp. and Clostridium sordellii were found both before and after pasteurization, but not after digestion (AD). Some of the isolated strains probably represented new members of the genera Clostridium and Bacillus. After digestion, the numbers of clostridia decreased, but none of the pathogenic bacteria did, whereas Bacillus spp. remained constant d...Continue Reading

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Citations

Sep 2, 2011·Current Infectious Disease Reports·Suzanne Zane, Jeannette Guarner
Jul 4, 2012·Applied and Environmental Microbiology·Aleksandra ChecinskaAndrzej J Paszczynski
Nov 7, 2013·Applied Microbiology and Biotechnology·Moez BoualiAbdelghani Sghir
Jul 6, 2014·Folia Microbiologica·Jürgen NeuhausMonika Krüger
Jun 9, 2012·The Science of the Total Environment·Lisa M AveryRupert L Hough
Nov 11, 2011·The Journal of General Virology·Hye Kwon KimBong Kyun Park
Jul 23, 2015·Journal of Applied Microbiology·B FröschleM Lebuhn

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