Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle

Microbiology
Maite MuniesaJuan Jofre

Abstract

The presence of bacteriophages encoding Shiga toxin 2 (stx(2) phages) was analysed in 168 strains of Shiga-toxin-producing Escherichia coli (STEC) isolated from cattle. Following mitomycin C induction, strains carrying stx(2) phages were screened by plaque blot and hybridization with an stx(2)A-probe. In the stx(2)-phage-carrying strains, the amounts of phage production, phage DNA extracted and Stx(2) produced after induction were assessed. The induced stx(2) phages were characterized morphologically and genetically. Assays to obtain lysogens from different strains were also carried out and phages induced from the lysogens were compared with those induced from the STEC isolates. Results indicated that 18 % of the strains carried an inducible stx(2) phage. Most of them showed a direct relationship between phage induction and toxin production. Each strain carried only one inducible stx(2) phage, although a few strains had two copies of the stx(2) in the chromosome. The stx(2) phages showed diverse morphology and a wide variability in their genome. Assays to obtain lysogens showed that not all the phages were transduced with the same frequency and only six lysogens were obtained. Phages in the lysogens were the same as those induc...Continue Reading

References

Jan 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·T MeyerJ Heesemann
Nov 25, 1988·Nucleic Acids Research·F Corpet
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Aug 26, 1998·Infection and Immunity·D W AchesonM K Waldor
Nov 18, 1998·Journal of Clinical Microbiology·A E HeuvelinkE de Boer
Feb 11, 2000·The Journal of Infectious Diseases·X ZhangD W Acheson
Mar 4, 2000·Applied and Environmental Microbiology·H SchmidtH Karch
Feb 27, 2001·The Journal of Antimicrobial Chemotherapy·S CruchagaM A Usera
Jun 16, 2001·International Journal of Food Microbiology·D HancockD Rice
Nov 28, 2002·Journal of Clinical Microbiology·Marjut EklundAnja Siitonen
Jun 5, 2003·Antonie van Leeuwenhoek·Maite MuniesaJuan Jofre
Feb 7, 2004·Molecular Biotechnology·Mohamed A Karmali
Mar 11, 2004·Molecular Microbiology·Jonathan Livny, David I Friedman

❮ Previous
Next ❯

Citations

Dec 22, 2005·Foodborne Pathogens and Disease·A D BrabbanT R Callaway
Feb 17, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Sheryl P Gow, Cheryl L Waldner
May 29, 2007·Antimicrobial Agents and Chemotherapy·Theresa J OchoaThomas G Cleary
Jul 14, 2010·Applied and Environmental Microbiology·Lejla ImamovicMaite Muniesa
Apr 17, 2012·Applied and Environmental Microbiology·Maite MuniesaHarald Brüssow
Jun 17, 2008·Applied and Environmental Microbiology·Musafiri Karama, Carlton L Gyles
Jun 12, 2013·Applied and Environmental Microbiology·Alexandre Martinez-CastilloMaite Muniesa
Jan 27, 2009·Applied and Environmental Microbiology·Lejla ImamovicMaite Muniesa
Mar 29, 2011·Applied and Environmental Microbiology·Lejla Imamovic, Maite Muniesa
Jan 1, 2013·Applied and Environmental Microbiology·Dongjin ParkCharles W Kaspar
Jan 5, 2011·Infection and Immunity·Cynthia A FullerAlison A Weiss
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien
Jul 18, 2012·BMC Genomics·Darren L SmithHeather E Allison
May 18, 2007·BMC Genomics·Yongxiang ZhangVictor P J Gannon
Mar 17, 2006·BMC Microbiology·Scot E Dowd, Hiroshi Ishizaki
Jun 16, 2012·PloS One·Neelanjana GhoshJordi Bella
Jun 7, 2014·Applied Microbiology and Biotechnology·Min Seok ChoDong Suk Park
Mar 23, 2011·Microbial Pathogenesis·Greg PetersonSanjeev Narayanan
Feb 1, 2012·Environmental Microbiology Reports·Alexandre Martínez-CastilloMaite Muniesa
Dec 3, 2015·Environmental Microbiology·Lejla ImamovicMaite Muniesa
Oct 30, 2009·Journal of Applied Microbiology·D DöpferA M Urdahl
Dec 7, 2014·Microbiology·Alejandra Krüger, Paula M A Lucchesi
Jun 9, 2015·Diagnostic Microbiology and Infectious Disease·Tanushree SaxenaMedicherla Krishna Mohan
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Domonkos SvábIstván Tóth
Dec 27, 2011·International Journal of Food Microbiology·Claudia PicozziRoberto Foschino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.