Diversity of the Gut Microbiota in Dihydrotestosterone-Induced PCOS Rats and the Pharmacologic Effects of Diane-35, Probiotics, and Berberine

Frontiers in Microbiology
Feifei ZhangCongjian Xu

Abstract

Polycystic ovary syndrome (PCOS) is a frequent endocrine and metabolic syndrome in reproductive-age women. Recently, emerging evidence has shown that gut microbiota is closely related to metabolic diseases such as type 2 diabetes, obesity and PCOS. In the present study, we established dihydrotestosterone (DHT)-induced PCOS rats and used Illumina MiSeq sequencing (PE300) to examine the composition, diversity, and abundance of the gut microbiota in PCOS. We compared the effects of three PCOS treatments: Diane-35 (estrogen and progesterone), probiotics and berberine. The DHT-induced rats showed constant estrous cycles, the loss of mature ovarian follicles, insulin resistance and obesity. The reproductive and metabolic functions in the PCOS rats were improved by treatment with Diane-35 and probiotics. Diane-35 and probiotics could restore the diversity of the gut microbiota, and the recovery of gut microbiota disorders improved the reproductive function in PCOS-like rats. However, berberine drastically reduced the species diversity and amount of gut microbiota and showed no improvement in PCOS. The findings of this study will help us to better understand the influence of the gut microbiota in the metabolic and reproductive alterati...Continue Reading

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Citations

Sep 13, 2019·Journal of the American College of Nutrition·Gamze Yurtdaş, Yasemin Akdevelioğlu
May 7, 2020·The ISME Journal·Hans K CarlsonAdam M Deutschbauer
Aug 30, 2020·The Journal of Clinical Endocrinology and Metabolism·Nesrin Damla EyupogluBulent Okan Yildiz
Nov 11, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lan WangLing Wang
Jan 8, 2021·Reproductive Sciences·Jingbo GuoJunping Hu
Mar 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Si-Wei ZhangLing Wang
Jun 1, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yuehui ZhangYanhua Han
Jan 28, 2022·Reproductive Sciences·Shikha Rani, Piyush Chandna

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Datasets Mentioned

BETA
SRP170100

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
PCR

Clinical Trials Mentioned

NCT02530385
NCT02741518

Software Mentioned

Trimmomatic
Clarity
UPARSE
Adonis
UCHIME
FLASH
SPSS

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