Diversity of Tn1546 and its role in the dissemination of vancomycin-resistant enterococci in Portugal

Antimicrobial Agents and Chemotherapy
Carla NovaisLuísa Peixe

Abstract

We characterized the molecular diversity of vanA vancomycin-resistant enterococci (VRE; 176 isolates/87 pulsed-field gel electrophoresis types) from different sources and cities in Portugal (1996 to 2004): (i) food animals (FA; n = 38 isolates out of 31 samples), hospitalized humans (HH; n = 101/101), healthy human volunteers (HV; n = 7/4), and environmental sources (n = 30/10). Some strains were isolated from different hosts and persistently recovered for years. Twenty-four Tn1546 variants were identified, all located on plasmids (30 to 250 kb). Some Tn1546 variants were associated with specific sources such as FA (3 types), HH (11 types), or HV (1 type), while others were recovered from isolates of different origins (8 types). Polymorphisms in the central vanRSHA region of Tn1546 were scarcely detected, while alterations upstream of vanR and downstream of vanA were frequently identified involving mutations (vanS and vanX), deletions (vanY), insertions (IS1216V, ISEf1, and IS19; sequences with or without homology with others available in GenBank databases), and different genetic rearrangements. Most Tn1546 variants contained IS1216V (14 types) or ISEf1 (6 types). IS1216V was found alone or associated with an IS3-like element a...Continue Reading

References

Apr 10, 1995·Analytical Biochemistry·B M BartonA J Zuccarelli
Feb 18, 1998·Journal of Clinical Microbiology·L B JensenF M Aarestrup
Mar 28, 1998·Antimicrobial Agents and Chemotherapy·N WoodfordB D Cookson
Dec 16, 1998·The Journal of Antimicrobial Chemotherapy·M F PalepouN Woodford
Feb 27, 1999·Antimicrobial Agents and Chemotherapy·R J WillemsJ D van Embden
Apr 14, 1999·Antimicrobial Agents and Chemotherapy·A L DariniN Woodford
Jul 17, 1999·Journal of Clinical Microbiology·G WernerW Witte
Aug 4, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·H de LencastreA Tomasz
Mar 2, 2002·The Lancet Infectious Diseases·M J BontenR A Weinstein
Mar 7, 2002·Microbes and Infection·Brett D Shepard, Michael S Gilmore
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Carla NovaisUNKNOWN Portuguese Resistance Study Group
May 25, 2005·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Henrik HasmanFrank M Aarestrup
Jun 4, 2005·Applied and Environmental Microbiology·Carla NovaisLuisa Peixe
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Carla NovaisUNKNOWN Portuguese Resistance Study Group
Oct 14, 2005·The Journal of Antimicrobial Chemotherapy·C NovaisL V Peixe
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patrice Courvalin
Apr 25, 2006·FEMS Microbiology Letters·Izabel Cristina V PalazzoAna Lúcia Costa Darini
Aug 2, 2006·Current Opinion in Microbiology·Helen L LeavisRob J L Willems
Sep 6, 2006·Journal of Bacteriology·Jonathan O'DriscollDouwe van Sinderen
Sep 28, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C NovaisL V Peixe
Jul 10, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M KawalecW Hryniewicz
Jul 20, 2007·Journal of Clinical Microbiology·Bo ZhengYasuyoshi Ike

❮ Previous
Next ❯

Citations

Jun 29, 2010·Foodborne Pathogens and Disease·Anette M HammerumOle E Heuer
Jan 20, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ting-Ting QuYun-Song Yu
Jun 15, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·María LópezCarmen Torres
Jun 4, 2010·The Journal of Antimicrobial Chemotherapy·María LópezCarmen Torres
Dec 7, 2010·The Journal of Antimicrobial Chemotherapy·Guido WernerArnfinn Sundsfjord
Aug 22, 2012·Antimicrobial Agents and Chemotherapy·Carla NovaisTeresa M Coque
Sep 25, 2008·Future Microbiology·Guido WernerWolfgang Witte
Jun 15, 2011·International Journal of Antimicrobial Agents·Ana R FreitasLuísa Peixe
Apr 10, 2010·Enfermedades infecciosas y microbiología clínica·Isabel MontesinosAntonio Sierra
Jun 3, 2015·The Journal of Antimicrobial Chemotherapy·Mette PinholtLotte Jakobsen
Sep 11, 2012·Diagnostic Microbiology and Infectious Disease·Leila Priscilla Pinheiro da SilvaAna Lúcia da Costa Darini
Apr 11, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A M Hammerum
Jun 24, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K HegstadA Sundsfjord
May 19, 2016·Chemosphere·Wenfang LinXin Yu
Oct 19, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E WardalE Sadowy
Oct 24, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohamed O Ahmed, Keith E Baptiste
May 4, 2016·Antimicrobial Agents and Chemotherapy·Audun SivertsenKristin Hegstad
Jan 31, 2019·Letters in Applied Microbiology·V OravcovaI Literak
Mar 4, 2020·The Journal of Antimicrobial Chemotherapy·Ingrid Maria Cecilia RubinMette Pinholt
Jan 22, 2021·Genome Medicine·Sergio Arredondo-AlonsoAnita C Schürch
Feb 23, 2020·Microorganisms·Valerie WistMagdalena Nüesch-Inderbinen
Jan 18, 2020·Microbiology Spectrum·Patrícia AntunesLuísa Peixe
Jul 28, 2018·Microbiology Spectrum·Carmen TorresTeresa M Coque
Aug 25, 2021·Journal of Global Antimicrobial Resistance·Michael BiggelRoger Stephan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.