Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi

Plant Molecular Biology
Richard G DorrellChristopher J Howe

Abstract

Plastids produce a vast diversity of transcripts. These include mature transcripts containing coding sequences, and their processing precursors, as well as transcripts that lack direct coding functions, such as antisense transcripts. Although plastid transcriptomes have been characterised for many plant species, less is known about the transcripts produced in other plastid lineages. We characterised the transcripts produced in the fucoxanthin-containing plastids of the dinoflagellate alga Karenia mikimotoi. This plastid lineage, acquired through tertiary endosymbiosis, utilises transcript processing pathways that are very different from those found in plants and green algae, including 3' poly(U) tail addition, and extensive substitutional editing of transcript sequences. We have sequenced the plastid transcriptome of K. mikimotoi, and have detected evidence for divergent evolution of fucoxanthin plastid genomes. We have additionally characterised polycistronic and monocistronic transcripts from two plastid loci, psbD-tRNA (Met)-ycf4 and rpl36-rps13-rps11. We find evidence for a range of transcripts produced from each locus that differ in terms of editing state, 5' end cleavage position, and poly(U) tail addition. Finally, we id...Continue Reading

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Citations

Aug 4, 2016·Molecular and Biochemical Parasitology·R E R Nisbet, J L McKenzie
Jul 28, 2016·Trends in Genetics : TIG·Gertraud BurgerMatus Valach
Apr 5, 2018·Genome Biology and Evolution·Christen M KlingerElisabeth Richardson
Aug 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth HehenbergerPatrick J Keeling
Jan 23, 2020·Genetics and Molecular Biology·Arpakorn SakulsathapornSupachai Vuttipongchaikij
Jan 19, 2021·Journal of Phycology·Richard G Dorrell

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Methods Mentioned

BETA
PCR
electrophoresis
PCRs

Software Mentioned

ORF Finder
ChloroP
Miseq
ASAfind
BLASTx
ARAGORN
BLAST
SignalP
tBLASTn

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