Mar 29, 2020

Pyocin efficacy in a murine model of Pseudomonas aeruginosa sepsis

BioRxiv : the Preprint Server for Biology
Madhur MangalamDaniel Walker

Abstract

Background: Bloodstream infections with antibiotic resistant Pseudomonas aeruginosa are common and increasingly difficult to treat. Pyocins are naturally occurring protein antibiotics produced by P. aeruginosa that have potential for human use. Objectives: To determine if pyocin treatment is effective in a murine model of sepsis with P. aeruginosa. Methods: Recombinant pyocins S5 and AP41 were purified tested for efficacy in a Galleria mellonella infection model and a murine model of P. aeruginosa sepsis. Results: Both pyocins produced no adverse effects when injected alone into mice and showed good in vitro antipseudomonal activity. In an invertebrate model of sepsis using Galleria mellonella, both pyocins significantly prolonged survival. Following injection into mice, both showed extensive distribution into different organs. When administered 5 hours after infection, both pyocins reduced mortality, with pyocin S5 being more effective than AP41. Conclusions: Pyocins S5 and AP41 show in vivo biological activity and can improve survival in a murine model of P. aeruginosa infection. They hold promise as novel antimicrobial agents for treatment of multi-drug resistant infections with this microbe.

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Mentioned in this Paper

Study
Capuchin Monkey
Patterns
2-Dimensional
Macaca radiata
Pseudo brand of pseudoephedrine
Research Personnel
Primates
Non-Human Protein
Species

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