Division patterns from single Escherichia coli cells

Biophysical Journal
W G Yeisley, E C Pollard

Abstract

A method has been worked out for studying the division of single bacterial cells (Escherichia coli B/r) in a uniform environment. Under optimal conditions the daughters of one single cell are found to divide at different times, a fact which indicates that they are not identical. The spread in generation times can be estimated quantitatively. When cells are irradiated with gamma rays in nutrient broth there is an increase in the spread in generation times, and the number of three-cell progeny (which require considerable difference between the daughters) rises. The results are consistent with the idea that damage to a segment of DNA has taken place and that there are three growing points on the DNA at any one time. In nutrient broth there is some evidence for repair of damage. For cells irradiated in minimal medium the pattern is different. The increase in generation times is not so marked, and only slight increase in three-cell progeny is seen. The results suggest that there is the same class of damage to the DNA, with only one growing point present and less repair. Using the criterion that no increase in generation time at all is permitted, we can estimate the total escape probability after radiation. The resulting calculation ...Continue Reading

References

Nov 1, 1962·Journal of General Microbiology·M SCHAECHTERA L KOCH
Mar 16, 1964·Biochimica Et Biophysica Acta·C E HELMSTETTER, D J CUMMINGS
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·H YOSHIKAWAN SUEOKA
Dec 1, 1964·Experimental Cell Research·J ENGELBERG
Mar 1, 1965·Journal of Molecular Biology·P F PACHLERM SCHAECHTER

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.