DivIVA affects secretion of virulence-related autolysins in Listeria monocytogenes.

Molecular Microbiology
Sven HalbedelAntje Flieger

Abstract

DivIVA is a well-conserved coiled-coil protein present in most Gram-positive bacteria and has been implicated in division site selection, peptidoglycan biosynthesis and sporulation. DivIVA proteins bind lipid membranes and characteristically accumulate at curved membrane areas, i.e. the cell poles and the division site, to which they recruit various interaction partners. We have studied the role of this morphogen in the human pathogen Listeria monocytogenes and our results suggest a novel mechanism by which DivIVA contributes to cell division. Contrary to expectation a ΔdivIVA mutant exhibited a pronounced chaining phenotype rather than a defect in cell division which we attributed to reduced extracellular levels of the autolytic enzymes p60 and MurA. We demonstrate that this is due to a malfunction in secretion of these autolysins and phenotypic comparison of the ΔdivIVA strain with a ΔsecA2 mutant suggests that DivIVA influences the activity of the SecA2 secretion route in L. monocytogenes. Also from the phenotypic analysis it was clear that divIVA affected swarming motility, biofilm formation, invasiveness and cell-to-cell spread in cell culture infection models. Thus, our experiments show that DivIVA is an important factor ...Continue Reading

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Citations

Dec 25, 2012·Journal of Bacteriology·Suey van BaarleSven Halbedel
Jan 8, 2013·Journal of Proteomics·Sandra RenierMickaël Desvaux
Jul 16, 2013·Environmental Microbiology·Orietta MassiddaWaldemar Vollmer
Sep 5, 2014·Molecular Microbiology·Karan Gautam KavalSven Halbedel
Oct 15, 2013·Molecular Microbiology·Boris SiegerMarc Bramkamp
Nov 5, 2013·Biochimica Et Biophysica Acta·Barbara A BensingPaul M Sullam
Nov 13, 2012·Current Opinion in Microbiology·Hélène Bierne, Shaynoor Dramsi
Nov 28, 2012·Current Opinion in Microbiology·Henrik Strahl, Leendert W Hamoen
May 9, 2014·Frontiers in Cellular and Infection Microbiology·Filipe CarvalhoDidier Cabanes
Jul 25, 2014·Molecular & Cellular Proteomics : MCP·Sven HalbedelAntje Flieger
Oct 4, 2017·Journal of Bacteriology·Karan Gautam KavalSven Halbedel
Oct 5, 2013·Clinical Microbiology Reviews·Romney M HumphriesGeorge Sakoulas
Sep 25, 2012·Nature Reviews. Microbiology·Meghan E Feltcher, Miriam Braunstein
Aug 14, 2019·Journal of Bacteriology·Lauren R HammondPrahathees J Eswara
Mar 20, 2019·Molecular Microbiology·Sven Halbedel, Richard J Lewis
Apr 5, 2018·Frontiers in Cellular and Infection Microbiology·Hua NiXiuzhen Pan
Mar 15, 2019·Emerging Microbes & Infections·Sven HalbedelAntje Flieger
Nov 25, 2020·Journal of Bacteriology·Jeanine RismondoAngelika Gründling
Jun 20, 2019·Microbiology Spectrum·Miriam BraunsteinPaul M Sullam

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