DivIVA concentrates mycobacterial cell envelope assembly for initiation and stabilization of polar growth

Cytoskeleton
Emily S MelzerM Sloan Siegrist

Abstract

In many model organisms, diffuse patterning of cell wall peptidoglycan synthesis by the actin homolog MreB enables the bacteria to maintain their characteristic rod shape. In Caulobacter crescentus and Escherichia coli, MreB is also required to sculpt this morphology de novo. Mycobacteria are rod-shaped but expand their cell wall from discrete polar or subpolar zones. In this genus, the tropomyosin-like protein DivIVA is required for the maintenance of cell morphology. DivIVA has also been proposed to direct peptidoglycan synthesis to the tips of the mycobacterial cell. The precise nature of this regulation is unclear, as is its role in creating rod shape from scratch. We find that DivIVA localizes nascent cell wall and covalently associated mycomembrane but is dispensable for the assembly process itself. Mycobacterium smegmatis rendered spherical by peptidoglycan digestion or by DivIVA depletion are able to regain rod shape at the population level in the presence of DivIVA. At the single cell level, there is a close spatiotemporal correlation between DivIVA foci, rod extrusion and concentrated cell wall synthesis. Thus, although the precise mechanistic details differ from other organisms, M. smegmatis also establish and propag...Continue Reading

References

Dec 1, 1979·Journal of General Microbiology·N Rastogi, T A Venkitasubramanian
Mar 1, 1987·Journal of General Microbiology·T OnodaA Matsuno
Mar 1, 1997·Journal of Bacteriology·J H Cha, G C Stewart
Apr 1, 1997·Anatomy and Embryology·P GunningP Jeffrey
May 1, 1997·Journal of Bacteriology·M A de PedroH Schwarz
Sep 7, 2001·Nature·F van den EntJ Löwe
Oct 24, 2003·Trends in Cell Biology·Rut Carballido-López, Jeff Errington
May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zemer GitaiLucy Shapiro
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kittichoat TiyanontSuzanne Walker
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kevin D Young
Sep 4, 2007·Journal of Bacteriology·Liem NguyenCharles J Thompson
May 30, 2009·The EMBO Journal·Rok LenarcicLeendert W Hamoen
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kumaran S Ramamurthi, Richard Losick
Nov 13, 2009·Current Biology : CB·William Margolin
Dec 22, 2009·Journal of Bacteriology·Constantin N TakacsChristine Jacobs-Wagner
May 7, 2010·Molecular Microbiology·Kerwyn Casey Huang, Kumaran S Ramamurthi
Nov 5, 2010·Annual Review of Genetics·Matthew T Cabeen, Christine Jacobs-Wagner
Dec 31, 2010·BMC Microbiology·Charul JaniChoong-Min Kang
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jun-Rong WeiEric J Rubin
Mar 23, 2011·BMC Research Notes·Graham JoyceKerstin J Williams
Jun 4, 2011·Science·Julia Domínguez-EscobarRut Carballido-López
Aug 10, 2011·Biochemistry·Marie H FossDouglas B Weibel
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sven van TeeffelenZemer Gitai
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Siyuan WangJoshua W Shaevitz
May 23, 2012·The Journal of Biological Chemistry·Renata PlocinskaMalini Rajagopalan
Sep 13, 2012·PloS One·Graham JoyceBrian D Robertson
Sep 18, 2012·Journal of the American Chemical Society·Benjamin M SwartsCarolyn R Bertozzi

❮ Previous
Next ❯

Citations

Sep 11, 2018·ELife·Alam García-HerediaM Sloan Siegrist
Aug 14, 2019·Journal of Bacteriology·Lauren R HammondPrahathees J Eswara
Jun 20, 2020·Communications Biology·Eveline UlteeAriane Briegel
Jan 12, 2021·Critical Reviews in Microbiology·Reema ChaudharyHari Misra
Feb 6, 2021·ELife·Alam García-HerediaM Sloan Siegrist
Apr 16, 2020·Journal of the American Chemical Society·Herbert W KavunjaBenjamin M Swarts
Nov 30, 2019·Journal of Structural Biology·Komal ChoukateBarnali Chaudhuri

❮ Previous
Next ❯

Software Mentioned

Fiji
Click
NIS

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.