DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway

Medical Oncology
Wei HuangCaiping Ren

Abstract

Loss of deleted in liver cancer-1 (DLC-1) can induce apoptosis and inhibit the mobility, migration and metastasis in several cancers. Previously, we revealed that ectopic expression of DLC-1 can suppress proliferation, mobility, migration and tumorigenesis in nasopharyngeal carcinoma (NPC). However, the molecular mechanisms accounting for the roles of DLC-1 in NPC are still obscure. In the present work, we attempted to study and uncover the mechanisms underlying the functions of DLC-1 in NPC. The apoptosis of 5-8F-DLC-1 cells, established previously, was analyzed by mitochondrial membrane potentials assay and flow cytometer analysis. And the antibodies involving pathways such as mitochondrial-associated apoptosis, epithelial mesenchymal transition and metastasis were applied to detect and compare the expression level of targeted proteins. The obvious apoptosis of 5-8F-DLC-1 cells was observed reflected by mitochondrial depolarization and lower ratio in cell viability. Subsequently, the activation of mitochondrial apoptosis was verified by the increased expressions of Bax, Apaf1, cleave-caspases and cleave-PARP, etc, and the decreased expressions of Bcl-2, Bcl-xL, Mcl-1, Survivin, etc, in 5-8F-DLC-1 cells. Then, the inhibited ep...Continue Reading

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Citations

Dec 5, 2017·Journal of the National Cancer Institute·David Sánchez-MartínGiovanna Tosato
Oct 18, 2016·Oncology Reports·Jianwei ZhuQifeng Guo
Nov 28, 2019·Journal of Cellular Physiology·Yang Zhang, Guorong Li
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Oct 24, 2020·Signal Transduction and Targeted Therapy·Yuanbo KangLei Wang
Jun 3, 2021·International Journal of Environmental Research and Public Health·Sheril June AnkashaNorfilza Mohd Mokhtar

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