Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia

Journal of Molecular Neuroscience : MN
M DingF M Vaccarino

Abstract

Homeodomain-containing genes of the Dlx family are expressed in the developing basal ganglia. To investigate the role of Dlx genes during development, we studied their cellular localization in primary cultures of embryonic basal telencephalon, and examined the changes in cellular phenotypes resulting from blockade of Dlx-2 expression. Cells containing Dlx-1, Dlx-2, and Dlx-5 mRNAs are immature cells of the neuronal lineage expressing the microtubule-associated proteins (MAPs) MAP1B and MAP2, but not glial fibrillary acidic protein (GFAP). Treatment of these cells with antisense oligonucleotides targeted to Dlx-2 caused a specific decrease of Dlx-2 mRNA and protein. This decrease in the Dlx-2 gene product was associated with a decrease in the expression of MAP2, a protein localized in neuronal dendrites, along with a smaller decrease in the 200-kDa neurofilament subunit (NF-H). Proteins expressed preferentially in axons were unchanged. This reduction in MAP2 expression was associated with a decrease in dendrite outgrowth and an increased level of cell proliferation. None of these changes were elicited by antisense oligonucleotides targeted to Dlx-1. We suggest that the Dlx-2 gene product regulates two interrelated aspects of neu...Continue Reading

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Citations

Apr 25, 2000·Gene·A J Bendall, C Abate-Shen
Jun 12, 2003·Molecular and Cellular Neurosciences·Gracie L AndrewsGrant S Mastick
Apr 14, 2012·Journal of Neuroendocrinology·R KapoorV A Vaidya
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Apr 17, 2021·Blood Cells, Molecules & Diseases·Yu-Wei WanXuan Liu

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