DMRT-1 expression during NEC8 human embryonic carcinoma cell differentiation

Cancer Science
Hitoshi KojiShinobu Gamou

Abstract

To elucidate the relationship between dsx and mab-3 related transcription factor 1 (Dmrt-1) and differentiation, alteration in mRNA levels during differentiation of NEC8 human embryonic carcinoma cells was investigated. After stimulation with 50 nM phorbol 12-myristate 13-acetate (PMA), the cells differentiated into cells with mesenchymal characteristics and upregulated Dmrt-1 mRNA, possibly through the protein kinase C/mitogen-activated protein kinase/activated protein-1 signaling pathway. Conversely, knockdown of Dmrt-1 by small interfering RNA resulted in cell morphology that was different from that after PMA treatment. These results indicated that Dmrt-1 expression was apparently associated with the differentiation of NEC8, and this cell line may be a helpful in vitro tool to clarify the role of Dmrt-1 in the differentiation process.

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Citations

Apr 18, 2013·PLoS Pathogens·Olivier TerrierManuel Rosa-Calatrava
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Naoki FujitaniYasuro Shinohara
Aug 28, 2007·Developmental Biology·Chang-Soo HongJean-Pierre Saint-Jeannet

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