DNA- and RNA-binding ability of oligoDapT, a nucleobase-decorated peptide, for biomedical applications

International Journal of Nanomedicine
Domenica MusumeciG Roviello

Abstract

Nucleobase-bearing peptides and their interaction with DNA and RNA are an important topic in the development of therapeutic approaches. On one hand, they are highly effective for modulating the nucleic-acid-based biological processes. On the other hand, they permit to overcome some of the main factors limiting the therapeutic efficacy of natural oligonucleotides, such as their rapid degradation by nucleases. This article describes the synthesis and characterization of a novel thymine-bearing nucleoamino acid based on the l-diaminopropionic acid (l-Dap) and its solid phase oligomerization to α-peptides (oligoDapT), characterized using mass spectrometry, spectroscopic techniques, and scanning electron microscopy (SEM) analysis. The interaction of the obtained nucleopeptide with DNA and RNA model systems as both single strands (dA12, rA12, and poly(rA)) and duplex structures (dA12/dT12 and poly(rA)/poly(rU)) was investigated by means of circular dichroism (CD) and ultraviolet (UV) experiments. From the analysis of our data, a clear ability of the nucleopeptide to bind nucleic acids emerged, with oligoDapT being able to form stable complexes with both unpaired and double-stranded DNA and RNA. In particular, dramatic changes in the ...Continue Reading

Citations

Mar 21, 2019·Anti-cancer Agents in Medicinal Chemistry·Heba A E Mohamed, Hossa F Al-Shareef
Feb 27, 2020·Chemical Reviews·Moran Frenkel-PinterLuke J Leman
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicola BorboneGiorgia Oliviero
May 20, 2020·Bioorganic Chemistry·Domenica MusumeciGiovanni N Roviello
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pasqualina Liana ScognamiglioGiovanni Nicola Roviello

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Methods Mentioned

BETA
circular dichroism
NMR
nuclear magnetic resonance

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