DNA-4'-6-diamidine-2-phenylindole interactions: a comparative study employing fluorescence and ultraviolet spectroscopy

Archives of Biochemistry and Biophysics
M L BarcellonaL Masotti

Abstract

DAPI is a drug that interacts with double-stranded nucleic acids, binding preferentially to A + T base pairs. The interaction is not intercalative, therefore providing a useful model for mimicking the effect of functional molecules in modifying specific sites, namely, A + T segments, of significance in gene expression. Knowledge of the nature of such interaction has been enriched by additional information obtained from comparative analysis of the data acquired by uv spectroscopy and fluorescence. Two classes of binding sites, defined by different apparent affinity constants and numbers of binding sites, are evident. All types of interaction are dependent on the nucleic acid/dye ratio and on the ionic strength of the medium.

References

Oct 15, 1976·Experimental Cell Research·D Schweizer
Aug 10, 1979·Nucleic Acids Research·J Kapuściński, W Szer
Jan 1, 1972·Biopolymers·D S StuddertR C Davis
Dec 20, 1983·Nucleic Acids Research·G ManziniF Quadrifoglio

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Citations

Jan 1, 1992·Biophysical Chemistry·A MazziniG Sartor
Dec 8, 1989·Biochimica Et Biophysica Acta·M L Barcellona, E Gratton
Jun 1, 1996·Journal of Biomolecular Structure & Dynamics·X MengD C Schwartz
Jun 28, 2000·Nucleic Acids Research·R M BroshV A Bohr
Apr 3, 2001·Applied and Environmental Microbiology·D K Button, B R Robertson
May 29, 1987·Biochemical and Biophysical Research Communications·G PaluG A Meloni
Feb 1, 1989·Analytical Biochemistry·M Katouzian-SafadiM Charlier
Jul 1, 1989·Cytometry·S Hamada, S Fujita
Sep 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J Kapuscinski
Aug 16, 2005·Biochemistry. Biokhimii︠a︡·V S SibirtsevV F Traven
Apr 1, 1997·Applied and Environmental Microbiology·S SabyJ C Block
Jan 9, 1996·Biochemistry·M L Barcellona, E Gratton

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