DNA amplification and chromosomal translocations are accompanied by chromosomal instability: analysis of seven human colon cancer cell lines by comparative genomic hybridization and spectral karyotyping

Cancer Genetics and Cytogenetics
T TsushimiK Sasaki

Abstract

Genetic instability in human cancers is classified as chromosomal instability (CIN) or microsatellite instability (MIN). DNA amplification and translocations are observed frequently in various cancers. We used comparative genomic hybridization (CGH) and spectral karyotyping (SKY) to study seven human colon cancer cell lines and investigate the relations among genetic instability, DNA amplification, and chromosomal translocations. DNA amplification was found in five cell lines (COLO320DM, COLO201, WiDr, CoCM-1, and CACO-2), and all were aneuploid. In these five cell lines, segments of chromosomes were translocated to other chromosomes. In contrast, cell lines with MIN, DLD-1, and LoVo did not show DNA amplification. The LoVo cells with MIN were considered near diploid and contained translocations. These findings suggest that DNA amplification and chromosomal translocations are accompanied by CIN.

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Citations

Nov 21, 2009·Genes, Chromosomes & Cancer·Turid KnutsenThomas Ried
Dec 2, 2004·Cancer Genetics and Cytogenetics·Kristine KleiviRagnhild A Lothe
Nov 7, 2002·Hematology/oncology Clinics of North America·John P Lynch, Timothy C Hoops
Oct 22, 2005·Medical Hypotheses·Steffen Hauptmann, Wolfgang D Schmitt
Dec 5, 2003·Oncogene·Jacques BertholonAlain Puisieux
May 28, 2019·Radiation and Environmental Biophysics·Felix ZwickerKlaus-Josef Weber
Jun 10, 2019·Investigational New Drugs·Jelena DinićJosé M Padrón
Feb 4, 2011·Cellular and Molecular Life Sciences : CMLS·Timo GemollJens K Habermann

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