PMID: 3771630Oct 1, 1986Paper

DNA amplification in multidrug, cross-resistant Chinese hamster ovary cells: molecular characterization and cytogenetic localization of the amplified DNA

The Journal of Cell Biology
L D TeeterT Kuo

Abstract

Vincristine-resistant (VCR) Chinese hamster ovary (CHO) cells have been established by stepwise selection in increasing concentrations of vincristine. These cells exhibit multidrug cross-resistance to a number of drugs that have no structural or functional similarities. Cytogenetic analyses of resistant cells revealed the presence of double minutes and expanded chromosomal segments, thus implicating gene amplification as a possible mechanism of resistance. An amplified DNA segment isolated from other multidrug cross-resistant CHO cell lines (Roninson, I. B., H. T. Abelson, D. E. Housman, N. Howell, and A. Varshavsky, 1984, Nature (Lond.), 309:626-628) is also amplified in our VCR lines. This DNA segment was used as a probe to screen a cosmid library of VCR genomic DNA, and overlapping clones were retrieved. All of these segments, totaling approximately 45 kilobases (kb), were amplified in VCR cells. Using in situ hybridization, we localized the amplification domain to the long arm of CHO chromosome 1 or Z1. Northern hybridization analysis revealed that a 4.3-kb mRNA was encoded by this amplified DNA domain and was over-produced in the VCR cells. Suggestions for the involvement of these amplified DNA segments in the acquisition ...Continue Reading

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Citations

Apr 20, 2001·Mechanisms of Ageing and Development·M SchiengoldN B Nardi
Jan 1, 1990·Journal of Neurosurgery·T MatsumotoJ Furuyama
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·M J SingerJ L Hamlin
Jan 1, 1988·European Journal of Haematology. Supplementum·E FricheK Danø
Aug 3, 1988·Biochimica Et Biophysica Acta·G BradleyV Ling

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