DNA analysis at p53 locus in adenoid cystic carcinoma: comparison of molecular study and p53 immunostaining

Pathology International
Y YamamotoA F Gazdar

Abstract

Abnormalities of the p53 tumor suppressor gene were investigated in 22 foci from 14 adenoid cystic carcinomas (ACC) by polymerase chain reaction (PCR)-based assays for dinucleotide (CA)n and pentanucleotide (AAAAT)n repeat polymorphisms and by immunohistochemical staining for oncoprotein expression. Adenoid cystic carcinomas were divided into lower grade (tubular and cribriform) subtypes and higher grade (trabecular and solid) subtypes. Histologically identified tumor cells were precisely microdissected from archival microslides and were used for molecular analysis. The overall frequency of p53 gene mutations detected by PCR-loss-of-heterozygosity (LOH) analysis was 57% and was higher than the frequency of over-expression of p53 oncoprotein detected by immunostaining (43%). In the molecular analysis of individual histological subtype foci, the number of foci with p53 gene mutation was significantly greater in the higher grade subtype foci than in the lower grade subtype foci and was greatest in solid-type foci (100%). In all six tumors in which histologically different foci were present in the same tumors, mutations of the p53 gene were detected. When tumor heterogeneity of the p53 gene was present among different histological ...Continue Reading

References

Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·Y SoiniP Pääkkö
May 14, 1992·The New England Journal of Medicine·A J Levine
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T SakaH Takahashi
Dec 25, 1991·Nucleic Acids Research·P A FutrealR W Wiseman
Jul 5, 1991·Science·M HollsteinC C Harris
Jun 6, 1991·Nature·A J LevineC A Finlay
May 1, 1972·Cancer·L S EbyH W Baker
Oct 1, 1970·American Journal of Surgery·S E ThorvaldssonJ N Simons
Jun 1, 1994·Genes, Chromosomes & Cancer·A NordkvistG Stenman
Jan 1, 1995·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Y YamamotoH Takahashi
Jun 28, 1995·JAMA : the Journal of the American Medical Association·J HungA F Gazdar
Feb 15, 1995·JAMA : the Journal of the American Medical Association·J HungA F Gazdar
Jan 1, 1995·Head & Neck·G FranzénO P Clausen
May 1, 1994·Human Pathology·H Battifora
Oct 1, 1961·Oral Surgery, Oral Medicine, and Oral Pathology·A P CHAUDHRYR J GORLIN

❮ Previous
Next ❯

Citations

Jan 1, 2010·BMJ Case Reports·I MasihR Convery
Jul 2, 2003·Journal of Clinical Pathology·M Pia-FoschiniS R Lakhani
Mar 16, 2002·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Angela D LevyCharles A Rohrmann
Aug 27, 2013·American Journal of Ophthalmology·Pia R MendozaJeffrey F Krane
Mar 18, 2008·Journal of the American Academy of Dermatology·Elizabeth NaylorLeslie Robinson-Bostom
Oct 19, 2011·Head & Neck·Jia LiuPatrick K Ha
Aug 5, 2015·Journal of Cutaneous Pathology·Laura D PozzobonJason K Wasserman
May 7, 2015·Oral Oncology·Andrés Coca-PelazAlfio Ferlito
Dec 31, 2014·Archives of Pathology & Laboratory Medicine·Shaobo ZhuJennifer Hunt
Feb 16, 2006·Brazilian Dental Journal·Luciana Fasanella Matizonkas-AntonioFabio Daumas Nunes
Dec 21, 2010·Advances in Anatomic Pathology·Raja R Seethala
Oct 16, 2002·Oncogene·Ignacio I WistubaAdi F Gazdar
Sep 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dong Sun LeeHak Soo Suh
Apr 12, 2008·Expert Review of Anticancer Therapy·Sreenivasa R Chandana, Barbara A Conley
Mar 21, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yongtao YuChristopher A Moskaluk
Jun 5, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hedy VékonyElisabeth Bloemena
Apr 30, 2011·Archives of Pathology & Laboratory Medicine·Jennifer L Hunt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Stine Rosenkilde LarsenAnnelise Krogdahl
APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
M H TherkildsenT Schiødt
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Hedy VékonyElisabeth Bloemena
© 2022 Meta ULC. All rights reserved