PMID: 9439148Jun 1, 1997Paper

DNA analysis by flow cytometry in early gastric cancer

The Korean Journal of Internal Medicine
J M KimS H Jung

Abstract

Flow cytometric analysis of a paraffin-embedded block of tissue provides rapid and accurate means of analyzing the DNA content of a tumor. The aim of this study was to clarify the clinical significance of flow cytometric findings in early gastric cancer(EGC). Thus we conducted this study to investigate whether DNA contents of tumor cells can correlate with known prognostic indices in patients with EGC. The flow cytometric DNA analysis was performed with paraffin-embedded specimens from tumors of 107 patients with EGC. Flow cytometric analysis was performed using a FACScan (Becton Dickinson). In constructing the histogram, 30,000 cells were scanned from each section and results were scored. The S-phase fraction was obtained according to the CellFit cell cycle analysis (Becton Dikinson). Frequencies of aneuploidy in tumors with various clinical and pathologic parameters were compared using the chi-square test. Mean SPF/PI valuse were compared by the student t-test. Diploidy pattern was observed in 80 (75%) cases while aneuploidy was seen in 27(25%) cases. Aneuploidy was more frequently detected in tumors with submucosal involvement (32.7%) and lymph node (+) group (30.8%) than in the mucosal tumor (17.3%) and lymph node (-) group...Continue Reading

References

Jun 1, 1989·Pathology, Research and Practice·K SasakiK Kawachnino
Jul 1, 1988·British Journal of Cancer·X de AretxabalaI Miyazaki
Apr 1, 1986·The Journal of Pathology·J C MacartneyG Powell
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Citations

Mar 10, 2012·Journal of Gastroenterology·Eiji OkiYoshihiko Maehara

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