PMID: 7536508Nov 1, 1994Paper

DNA and keratin analysis of oral exfoliative cytology in the detection of oral cancer

European Journal of Cancer. Part B, Oral Oncology
G R OgdenE B Lane

Abstract

Refinements in oral exfoliative cytology may make it a suitable screening technique for the early diagnosis of oral cancer. In this study DNA range profiles were combined with keratin expression in an attempt to improve the diagnostic accuracy of oral exfoliative cytology. Smears were taken from 33 biopsy-proven oral cancers and the contralateral normal site. For DNA range profiles the smears underwent Feulgen hydrolysis, with DNA distribution being assessed using the Vickers M85 microdensitometer. For keratin expression a panel of antikeratin antibodies were applied. The smears for keratin expression were then graded on a three-point scale. Abnormal DNA range profiles were observed in 23 of 33 smears taken from oral cancers and in two smears from normal oral mucosa (sensitivity 70%, specificity 90%, positive predictive value 90%). The simple epithelial keratins 8 and 19 were identified in the majority of oral cancer smears. The sensitivity of keratin 19 was greater (90%). However, keratin 8 was the most useful keratin marker associated with malignancy (sensitivity 62%, specificity 100%, positive predictive value 100%). The combination of simple keratin expression and DNA content improved the cancer detection rate beyond that o...Continue Reading

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Citations

Jan 25, 2008·HNO·O DriemelH Kosmehl
Sep 1, 1996·European Journal of Cancer. Part B, Oral Oncology·G R Ogden
May 1, 1997·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G R OgdenA J Wight
Jun 5, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Deepika ShuklaYerrmalla Venkatakanthaiah
Dec 21, 2006·Mund-, Kiefer- und Gesichtschirurgie : MKG·M HullmannO Driemel
Nov 23, 2007·Cytopathology : Official Journal of the British Society for Clinical Cytology·O DriemelH Kosmehl
Oct 1, 1996·The British Journal of Oral & Maxillofacial Surgery·G R OgdenE B Lane
Feb 13, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·R MehrotraO Driemel
Apr 30, 2010·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Pérez-SayánsA García-García
Apr 30, 2011·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Pérez-SayánsA García-García
Aug 21, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Llanos CasanovaJosé L Jorcano
Jul 21, 2021·The Cochrane Database of Systematic Reviews·Tanya WalshSaman Warnakulasuriya

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