PMID: 2493444Mar 1, 1989Paper

DNA and protein sequence conservation at the replication terminus in Bacillus subtilis 168 and W23

Journal of Bacteriology
P J Lewis, R G Wake

Abstract

Cloned DNA from the replication terminus region of Bacillus subtilis 168 was used to identify and construct a restriction map of the homologous region in B. subtilis W23. With this information, DNA from the terminus region of W23 was cloned and the sequence was determined for a 1,499-base-pair segment spanning the expected terC site. The position of the site was then located more precisely. Use of the cloned DNA from strain W23 as a probe for digests of DNA from exponentially growing cells of the same strain established the presence of the slowly migrating replication termination intermediate (forked DNA). The orientation and dimensions of the forked molecule were consistent with arrest of the clockwise fork at the terC site in W23, as has been shown to occur in strain 168. Thus, despite significant differences between the two strains, the same termination mechanism appears to be used. The DNA sequences spanning the terC site in strains 168 and W23 showed a high level of homology (90.2%) close to the site but very little at a distance of approximately 250 base pairs from the site in one particular direction. The overall sequence comparison emphasised the importance of the open reading frame for a 122-amino-acid protein adjacent...Continue Reading

References

Mar 1, 1975·Journal of Bacteriology·N Harford
Sep 1, 1975·Bacteriological Reviews·H E Hemphill, H R Whiteley
Sep 1, 1988·Journal of Bacteriology·M T Smith, R G Wake
Mar 20, 1986·Journal of Molecular Biology·A S WeissR B Inman
Oct 26, 1987·Nucleic Acids Research·C M CarriganR G Wake
Nov 1, 1969·Journal of Bacteriology·P S Lovett, F E Young
Dec 5, 1983·Journal of Molecular Biology·A S Weiss, R G Wake
Apr 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·H YOSHIKAWA, N SUEOKA
Jun 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·H YOSHIKAWA, N SUEOKA

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Citations

Sep 1, 1991·Research in Microbiology·P J Lewis, R G Wake
May 1, 1997·Journal of Bacteriology·A A Griffiths, R G Wake

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