DNA aptamer folding on magnetic beads for sequential detection of adenosine and cocaine by substrate-resolved chemiluminescence technology

The Analyst
Xiluan YanJianzhong Lu

Abstract

We have developed a new aptamer-based chemiluminescence (CL) biosensing platform for the sequential detection of two small molecules as exemplified by adenosine and cocaine. Each biosensing platform comprises NH(2)-functionalized capture DNA immobilized on magnetic beads; this can hybridize with one end of the aptamer. A corresponding reporter DNA probe labeled with either digoxigenin or biotin on its 5'-terminus recognizes the other end of the aptamer. The target compounds adenosine or cocaine act as specific competitors to aptamer-reporter DNA binding, and the corresponding aptamers preferentially form target-aptamer complexes. This results in detachment of the reporter DNA probe from the magnetic beads, with more target molecules resulting in less reporter DNA probe remaining on the beads. Those left are sequentially detected by using substrate-resolved anti-digoxigenin-alkaline phosphatase and streptavidin-horseradish peroxidase. Experimental results confirm that this CL immunosensing platform has good sensitivity with detection limits of 5.2 x 10(-9) M and 3.2 x 10(-9) M for adenosine and cocaine, respectively. Because it is straightforward to adapt this strategy to detect a spectrum of small molecules by using different a...Continue Reading

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Citations

Jul 25, 2012·Analytical and Bioanalytical Chemistry·Kris P F JanssenJeroen Lammertyn
Jan 29, 2013·Biosensors & Bioelectronics·Johannes DapràNoemi Rozlosnik
Apr 29, 2011·Analytical Chemistry·Anton B IliukW Andy Tao
Oct 19, 2012·Journal of Clinical Microbiology·Manoosak WongphatcharachaiSrinand Sreevatsan
Aug 1, 2012·Expert Review of Molecular Diagnostics·Yue Zhang, Dejian Zhou
Mar 23, 2011·Biosensors & Bioelectronics·Audrey SassolasBéatrice D Leca-Bouvier
Feb 22, 2012·Analytica Chimica Acta·Jinwen ZhouNicolas H Voelcker
Apr 3, 2014·Biosensors & Bioelectronics·Chunjing FengLei Wang
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahmoud Roushani, Faezeh Shahdost-Fard
Apr 7, 2018·The Analyst·Jahangir Ahmad RatherPalanisamy Kannan
Nov 1, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Yue Sun, Jianzhong Lu

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Methods Mentioned

BETA
biosensing
biosensor
aptasensor

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