DNA aptamers block L-selectin function in vivo. Inhibition of human lymphocyte trafficking in SCID mice

The Journal of Clinical Investigation
B J HickeD Parma

Abstract

Selectins participate in the initial events leading to leukocyte extravasation from the blood into tissues. Thus the selectins have generated much interest as targets for antiinflammatory agents. Therapeutic molecules based on the monomeric carbohydrate ligand sialyl Lewis X (SLe(X)) have low affinities and are not specific for a given selectin. Using SELEX (Systematic Evolution of Ligands by EXponential Enrichment) technology, we have generated aptamers specific for L-selectin that require divalent cations for binding and have low nanomolar affinity. In vitro, the deoxyoligonucleotides inhibit L-selectin binding to immobilized SLe(X) in static assays and inhibit L-selectin-mediated rolling of human lymphocytes and neutrophils on cytokine-activated endothelial cells in flow-based assays. These aptamers also block L-selectin-dependent lymphocyte trafficking in vivo, indicating their potential utility as therapeutics.

References

Jan 1, 1992·Annual Review of Immunology·L J Picker, E C Butcher
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T K KishimotoE C Butcher
Jan 1, 1994·Annual Review of Medicine·M P BevilacquaO Cecconi
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Oct 1, 1994·Current Opinion in Cell Biology·S D Rosen, C R Bertozzi
May 12, 1995·The Journal of Biological Chemistry·R P McEverR D Cummings
Jan 1, 1995·Annual Review of Biochemistry·L GoldM Yarus
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Mar 1, 1993·The Journal of Cell Biology·D V ErbeL A Lasky
Mar 1, 1993·The Journal of Clinical Investigation·R M NelsonM P Bevilacqua
Oct 15, 1993·Science·S BaumheterL A Lasky
Jun 9, 1995·The Journal of Biological Chemistry·L Gold
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·D O'ConnellA Varki
Jun 27, 1949·Annals of the New York Academy of Sciences·E J KEPLER

❮ Previous
Next ❯

Citations

May 15, 2002·Electrophoresis·Stacey L Clark, Vincent T Remcho
Jun 22, 2000·Analytical Biochemistry·M Lee, D R Walt
Sep 25, 2010·Analytical and Bioanalytical Chemistry·Zhenyu ShaoGenxi Li
Dec 17, 2010·Analytical and Bioanalytical Chemistry·Boonchoy Soontornworajit, Yong Wang
Jun 21, 2005·Biochimie·C PestourieF Duconge
Mar 9, 2004·Methods : a Companion to Methods in Enzymology·Narendra K VaishScott D Seiwert
Sep 19, 1998·Journal of the Neurological Sciences·J J ArchelosH P Hartung
Jun 6, 2000·Advanced Drug Delivery Reviews·M Willis, E Forssen
Jun 27, 2002·Peptides·Insug OMagdalena Blaszczyk-Thurin
May 1, 1997·Molecular Medicine Today·H RossiterT S Kupper
Jun 12, 2010·Nature Protocols·Kwame SefahWeihong Tan
May 14, 2008·Human Gene Therapy·Claudia M DollinsBruce A Sullenger
Sep 4, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Custer C DeocarisRenu Wadhwa
Jun 10, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weian ZhaoJeffrey M Karp
Aug 7, 2009·Journal of Biomechanical Engineering·Todd O PangburnEfrosini Kokkoli
Oct 8, 2009·Molekuliarnaia biologiia·E R SurinaS V Benevolenskiĭ
Jun 29, 2000·Annual Review of Biochemistry·D S Wilson, J W Szostak
Jan 15, 1997·The Journal of Clinical Investigation·A Varki
Mar 1, 1997·The Journal of Clinical Investigation·J B Lowe, P A Ward
Sep 10, 2011·Journal of Nucleic Acids·Cindy MeyerAndrea Rentmeister
Feb 17, 2001·Biochemical and Biophysical Research Communications·S JeongJ Yu
Aug 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steffen HelmlingSven Klussmann
Apr 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rebekah R WhiteBruce A Sullenger
Sep 1, 2007·Expert Opinion on Drug Discovery·Aniela WochnerMartina Rimmele
Jul 25, 2006·Expert Opinion on Investigational Drugs·Daniel BockGerhard Wolff
Jun 5, 2014·Biotechnology and Bioengineering·Eric OuelletCharles A Haynes
Jan 17, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Sebastian B RieseJens Dernedde
Jan 8, 2004·The Journal of Investigative Dermatology·Michael P SchönW Henning Boehncke
Jul 14, 2007·Biomolecular Engineering·Regina StoltenburgBeate Strehlitz
Nov 26, 2015·International Immunopharmacology·Mostafa KhedriMohammad Ramezani
Jan 8, 2014·Proteomics·Bridget LolloLarry Gold
Mar 18, 2016·The Protein Journal·Maryam Tabarzad, Marzieh Jafari
Apr 28, 2005·Biosensors & Bioelectronics·S TombelliM Mascini
Jul 8, 2015·Current Opinion in Immunology·Judy Lieberman
Apr 1, 2015·PloS One·Elizabeth K ChangWeian Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D O'ConnellA Varki
© 2022 Meta ULC. All rights reserved