DNA barcode authentication of saw palmetto herbal dietary supplements

Scientific Reports
Damon P Little, Marc L Jeanson

Abstract

Herbal dietary supplements made from saw palmetto (Serenoa repens; Arecaceae) fruit are commonly consumed to ameliorate benign prostate hyperplasia. A novel DNA mini-barcode assay to accurately identify [specificity = 1.00 (95% confidence interval = 0.74-1.00); sensitivity = 1.00 (95% confidence interval = 0.66-1.00); n = 31] saw palmetto dietary supplements was designed from a DNA barcode reference library created for this purpose. The mini-barcodes were used to estimate the frequency of mislabeled saw palmetto herbal dietary supplements on the market in the United States of America. Of the 37 supplements examined, amplifiable DNA could be extracted from 34 (92%). Mini-barcode analysis of these supplements demonstrated that 29 (85%) contain saw palmetto and that 2 (6%) supplements contain related species that cannot be legally sold as herbal dietary supplements in the United States of America. The identity of 3 (9%) supplements could not be conclusively determined.

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Citations

Jun 17, 2015·Plant Biotechnology Journal·Priyanka MishraVelusamy Sundaresan
Dec 3, 2014·Gene·Bishal Dhar, Sankar Kumar Ghosh
Jun 4, 2011·PloS One·Peter M HollingsworthDamon P Little
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May 28, 2020·Scientific Reports·Morgan R GostelCaroline Puente-Lelievre
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Datasets Mentioned

BETA
AJ621936
M81815

Methods Mentioned

BETA
PCR

Software Mentioned

Sequencher
BRONX
KB
matK
MUSCLE
PRIMER3

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