PMID: 30156397Jun 1, 2016Paper

DNA Barcode Screening and Identifying for Anredera cordifolia and Its Related Species

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Li-ying YangShu-Yuan Li

Abstract

To identify Anredera cordifolia and its closely related species using the DNA barcode. 28 individuals of Anredera cordifolia and its close related species were collected from different habitats. ITS and ITS2 of ribosomal DNA,matK,rbcL and psb A-trn H of chloroplast DNA were amplified and sequenced. The amplification and sequencing success rate,barcoding gap,and NJ trees were used to evaluate the efficiency of species identification. After amplified and sequenced, base deletion was occurred in psb A-trnH sequences of Basella alba. The sequencing success rates of mat K,rbc L,ITS and ITS2 were 100%,100%,78. 75% and64. 28%,respectively. Among the four DNA barcoding sequences,ITS and mat K had remarkable barcoding gap. The NJ tree showed that Anredera cordifolia could differed obviously from its closely related species by ITS and mat K. The sequences of ITS and matK provide an effective and fast tool for the identification and authentication of medicinal plant of Anredera cordifolia and its related species.

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Yao xue xue bao = Acta pharmaceutica Sinica
Yingjie ZhuJing Lu
Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ning YuAidong Sun
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Zhen LiuShilin Chen
Yao xue xue bao = Acta pharmaceutica Sinica
Shan-Shan FengLinfang Huang
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Huan TangMeng Ye
© 2022 Meta ULC. All rights reserved