DNA barcoding Brooklyn (New York): A first assessment of biodiversity in Marine Park by citizen scientists

PloS One
Christine MarizziDavid Andrew Micklos

Abstract

DNA barcoding is both an important research and science education tool. The technique allows for quick and accurate species identification using only minimal amounts of tissue samples taken from any organism at any developmental phase. DNA barcoding has many practical applications including furthering the study of taxonomy and monitoring biodiversity. In addition to these uses, DNA barcoding is a powerful tool to empower, engage, and educate students in the scientific method while conducting productive and creative research. The study presented here provides the first assessment of Marine Park (Brooklyn, New York, USA) biodiversity using DNA barcoding. New York City citizen scientists (high school students and their teachers) were trained to identify species using DNA barcoding during a two-week long institute. By performing NCBI GenBank BLAST searches, students taxonomically identified 187 samples (1 fungus, 70 animals and 116 plants) and also published 12 novel DNA barcodes on GenBank. Students also identified 7 ant species and demonstrated the potential of DNA barcoding for identification of this especially diverse group when coupled with traditional taxonomy using morphology. Here we outline how DNA barcoding allows citizen...Continue Reading

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Citations

Jul 3, 2021·International Journal of Environmental Research and Public Health·Ayat AbourashedEric C M van Gorp

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Datasets Mentioned

BETA
KU682771
KU682774
KU682775

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

DNA
DNA Subway
Subway
DNA Subway sequence viewer
BLAST
Phred

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