DNA Barcoding for the Identification and Authentication of Animal Species in Traditional Medicine

Evidence-based Complementary and Alternative Medicine : ECAM
Fan YangHaifeng Li

Abstract

Animal-based traditional medicine not only plays a significant role in therapeutic practices worldwide but also provides a potential compound library for drug discovery. However, persistent hunting and illegal trade markedly threaten numerous medicinal animal species, and increasing demand further provokes the emergence of various adulterants. As the conventional methods are difficult and time-consuming to detect processed products or identify animal species with similar morphology, developing novel authentication methods for animal-based traditional medicine represents an urgent need. During the last decade, DNA barcoding offers an accurate and efficient strategy that can identify existing species and discover unknown species via analysis of sequence variation in a standardized region of DNA. Recent studies have shown that DNA barcoding as well as minibarcoding and metabarcoding is capable of identifying animal species and discriminating the authentics from the adulterants in various types of traditional medicines, including raw materials, processed products, and complex preparations. These techniques can also be used to detect the unlabelled and threatened animal species in traditional medicine. Here, we review the recent pro...Continue Reading

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Citations

Oct 5, 2019·Chinese Medicine·Xiaoxiao ZhuWeiwei Su
Jan 9, 2019·Frontiers in Plant Science·Tiziana SgammaAdrian Slater
Apr 9, 2020·Frontiers in Pharmacology·Xinyun CuiXilong Zheng
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Feb 9, 2021·Genome Génome / Conseil National De Recherches Canada·Fátima Yedith Camacho-SánchezMiguel Angel Reyes-López

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Methods Mentioned

BETA
PCR
amplicon sequencing

Software Mentioned

Barcode Index Number System ( BIN
Basic Local Alignment Search Tool ( BLAST )
Primer Premier
MEGA
PHYLIP
Oligo
Barcode of Life Data System ( BOLD
PAUP

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