DNA barcoding of fungi causing infections in humans and animals

Fungal Biology
Laszlo IrinyiWieland Meyer

Abstract

Correct species identification is becoming increasingly important in clinical diagnostics. Till now, many mycological laboratories rely on conventional phenotypic identification. But this is slow and strongly operator-dependent. Therefore, to improve the quality of pathogen identification, rapid, reliable, and objective identification methods are essential. One of the most encouraging approaches is molecular barcoding using the internal transcribed spacer (ITS) of the rDNA, which is rapid, easily achievable, accurate, and applicable directly from clinical specimens. It relies on the comparison of a single ITS sequence with a curated reference database. The International Society for Human and Animal Mycology (ISHAM) working group for DNA barcoding has recently established such a database, focusing on the majority of human and animal pathogenic fungi (ISHAM-ITS, freely accessible at http://www.isham.org/ or directly from http://its.mycologylab.org). For some fungi the use of secondary barcodes may be necessary.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1990·Current Genetics·D Vilgalys, D Gonzalez
Jul 1, 1997·Molecular Biology and Evolution·Z Yang, B Rannala
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·J W FellA Statzell-Tallman
Dec 16, 2000·Fungal Genetics and Biology : FG & B·J W TaylorM C Fisher
May 31, 2002·Journal of Clinical Microbiology·Alain DefontaineJean-Philippe Bouchara
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Sep 4, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Jan 30, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C Dignani, E Anaissie
Apr 9, 2005·Nature·Malte C Ebach, Craig Holdrege
Aug 6, 2005·Mycological Research·Alexey KopchinskiyIrina S Druzhinina
Nov 30, 2005·The Journal of General and Applied Microbiology·Yuuhiko TanabeJunta Sugiyama
Dec 13, 2005·PLoS Biology·Christopher P Meyer, Gustav Paulay
Mar 7, 2006·Journal of Clinical Microbiology·Shiang Ning LeawTsung Chain Chang
Feb 24, 2007·Trends in Genetics : TIG·Mehrdad HajibabaeiDonal A Hickey
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Keith A SeifertPaul D N Hebert
Jun 19, 2007·Mycological Research·David S HibbettNing Zhang
Jan 19, 2008·Clinical Microbiology Reviews·Karoll J CortezThomas J Walsh
Mar 22, 2008·Science·M I Bidartondo
May 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ben De PauwUNKNOWN National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group
May 21, 2008·Studies in Mycology·D M GeiserR A Samson
May 21, 2008·Studies in Mycology·S A BalajeeR A Samson
Sep 12, 2008·Molecular Ecology Notes·Sujeevan Ratnasingham, Paul D N Hebert
Nov 26, 2008·Medical Mycology·Juan Luis Rodriguez-TudelaManuel Cuenca-Estrella
May 23, 2009·FEMS Microbiology Letters·Rolf Henrik NilssonErik Kristiansson
Jun 26, 2009·BMC Bioinformatics·Monica SantamariaCecilia Saccone
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·UNKNOWN CBOL Plant Working Group
Sep 29, 2009·Trends in Microbiology·David J McLaughlinRytas Vilgalys
Oct 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·W John KressEldredge Bermingham
Jan 1, 2010·Journal of Clinical Microbiology·Anna M RomanelliBrian L Wickes
Apr 21, 2010·Applied Microbiology and Biotechnology·Dominik BegerowWolfgang Maier
Aug 6, 2010·Journal of Clinical Microbiology·Kerry O'DonnellDavid M Geiser
May 1, 2009·Molecular Ecology Resources·Keith A Seifert
May 1, 2009·Molecular Ecology Resources·Scott R GilmoreKeith A Seifert
May 1, 2009·Molecular Ecology Resources·Agathe VialleRichard C Hamelin
Sep 3, 2011·PLoS Biology·Camilo MoraBoris Worm
Oct 4, 2011·PloS One·Bryn T M DentingerJean-Marc Moncalvo

❮ Previous
Next ❯

Citations

Nov 11, 2016·Genome Génome / Conseil National De Recherches Canada·Jianping Xu
Feb 16, 2017·Journal of Natural Products·Huzefa A RajaNicholas H Oberlies
Dec 19, 2018·Veterinary Pathology·Sunil Nivrutti MoreWilliam L Castleman
Apr 5, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Pierre BeckerMarijke Hendrickx
Aug 6, 2019·Frontiers in Microbiology·Minh Thuy Vi HoangWieland Meyer
Dec 1, 2017·JMM Case Reports·Joanna LodzinskaGavin K Paterson
Jun 22, 2019·Journal of Clinical Immunology·F Queiroz-TellesAnete S Grumach
Jan 19, 2020·Microorganisms·Carla ViegasAnn Marie Coggins
Apr 2, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·B NyuykongeW W J van de Sande
Jan 30, 2020·Frontiers in Microbiology·Sarah E KiddCatriona L Halliday
May 23, 2020·Emerging Infectious Diseases·Tahinamandranto RasamoelinaLala S Ramarozatovo
Sep 15, 2017·Indian Journal of Microbiology·Richa BafanaR A Pandey
Sep 27, 2018·Frontiers in Genetics·Pria N GhoshKieran A Bates
Oct 13, 2019·Mycopathologia·Ann PackeuMarijke Hendrickx
Sep 21, 2019·Emerging Infectious Diseases·Tahinamandranto RasamoelinaMuriel Cornet
Sep 11, 2018·Ophthalmic Plastic and Reconstructive Surgery·Kyle J GodfreyMichael Kazim
Nov 21, 2020·Journal of Fungi·Morgana Ferreira VoidaleskiVânia Aparecida Vicente
Feb 16, 2021·Veterinary Pathology·Alexandra N MyersAline Rodrigues Hoffmann
Jun 4, 2019·FEMS Microbiology Reviews·UNKNOWN Consortium OPATHY, Toni Gabaldón
Oct 12, 2016·Microbiology Spectrum·Sean X Zhang, Nathan P Wiederhold
May 18, 2021·Forensic Science International : Synergy·Yanlei LiuShiliang Zhou
Aug 27, 2021·Journal of Fungi·Yahaya HassanLeslie Thian Lung Than
Sep 8, 2021·Mycopathologia·Amelie P BrackinJohanna Rhodes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.