DNA barcoding of Rhodiola (crassulaceae): a case study on a group of recently diversified medicinal plants from the Qinghai-Tibetan Plateau

PloS One
Jian-Qiang ZhangGuang-Yuan Rao

Abstract

DNA barcoding, the identification of species using one or a few short standardized DNA sequences, is an important complement to traditional taxonomy. However, there are particular challenges for barcoding plants, especially for species with complex evolutionary histories. We herein evaluated the utility of five candidate sequences - rbcL, matK, trnH-psbA, trnL-F and the internal transcribed spacer (ITS) - for barcoding Rhodiola species, a group of high-altitude plants frequently used as adaptogens, hemostatics and tonics in traditional Tibetan medicine. Rhodiola was suggested to have diversified rapidly recently. The genus is thus a good model for testing DNA barcoding strategies for recently diversified medicinal plants. This study analyzed 189 accessions, representing 47 of the 55 recognized Rhodiola species in the Flora of China treatment. Based on intraspecific and interspecific divergence and degree of monophyly statistics, ITS was the best single-locus barcode, resolving 66% of the Rhodiola species. The core combination rbcL+matK resolved only 40.4% of them. Unsurprisingly, the combined use of all five loci provided the highest discrimination power, resolving 80.9% of the species. However, this is weaker than the discrimi...Continue Reading

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Citations

Dec 2, 2018·Future Medicinal Chemistry·Da-Cheng HaoChang Liu
Dec 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Abhijit Dey
Mar 19, 2021·Food Science and Biotechnology·Kwan Joong KimDae-Ok Kim

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
MUSCLE
Modeltest
MulTrees
Geneious
ContigExpress
Vector NTI Suite
RAxML
MEGA
TaxonDNA

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