Developing molecular communication platforms based on orthogonal communication channels is a crucial step towards engineering artificial multicellular systems. Here, we present a general and scalable platform entitled 'biomolecular implementation of protocellular communication' (BIO-PC) to engineer distributed multichannel molecular communication between populations of non-lipid semipermeable microcapsules. Our method leverages the modularity and scalability of enzyme-free DNA strand-displacement circuits to develop protocellular consortia that can sense, process and respond to DNA-based messages. We engineer a rich variety of biochemical communication devices capable of cascaded amplification, bidirectional communication and distributed computational operations. Encapsulating DNA strand-displacement circuits further allows their use in concentrated serum where non-compartmentalized DNA circuits cannot operate. BIO-PC enables reliable execution of distributed DNA-based molecular programs in biologically relevant environments and opens new directions in DNA computing and minimal cell technology.
Contextualizing context for synthetic biology--identifying causes of failure of synthetic biological systems
Interfacial assembly of protein-polymer nano-conjugates into stimulus-responsive biomimetic protocells
Construction of biological hybrid microcapsules with defined permeability towards programmed release of biomacromolecules
Artificial intelligence exploration of unstable protocells leads to predictable properties and discovery of collective behavior
Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens
Invasion and Defense Interactions between Enzyme-Active Liquid Coacervate Protocells and Living Cells.
Chemical communication in spatially organized protocell colonies and protocell/living cell micro-arrays
Intercellular communication between artificial cells by allosteric amplification of a molecular signal.
Functional and morphological adaptation in DNA protocells via signal processing prompted by artificial metalloenzymes.
Cell-free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices
Formation of Polarized, Functional Artificial Cells from Compartmentalized Droplet Networks and Nanomaterials, Using One-Step, Dual-Material 3D-Printed Microfluidics
ATP-powered molecular recognition to engineer transient multivalency and self-sorting 4D hierarchical systems
Signaling in Systems Chemistry: Programing Gold Nanoparticles Formation and Assembly Using a Dynamic Bistable Network.
In search of a novel chassis material for synthetic cells: emergence of synthetic peptide compartment
Enzyme-mediated nitric oxide production in vasoactive erythrocyte membrane-enclosed coacervate protocells.
Deformation dynamics of giant unilamellar vesicles in the large surface-to-volume ratio regime: the emergence of neuron-like morphology
A Modular, Dynamic, DNA-Based Platform for Regulating Cargo Distribution and Transport between Lipid Domains.
Microscopic Imaging Techniques for Molecular Assemblies: Electron, Atomic Force, and Confocal Microscopies.
Autocatalytic and oscillatory reaction networks that form guanidines and products of their cyclization.
Artificial Organelles with Orthogonal-Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin-Polymersomes.
Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.