DNA-based identification of forensically important Lucilia (Diptera: Calliphoridae) in the continental United States

Journal of Forensic Sciences
Ronald W DebryGregory A Dahlem

Abstract

Correct species identification is critical when dipteran larvae are used for inference of the postmortem interval. To facilitate DNA-based identification of forensically important flies of the genus Lucilia in the continental United States, we develop a vouchered reference collection and DNA sequence database. A total of 122 specimens were collected for nine of the 10 species of Lucilia reported to occur in the continental United States. Using the polymerase chain reaction and DNA sequencing, data were obtained for an 1100-bp region of the mitochondrial gene encoding cytochrome oxidase I (COI). We consider a species suitable for DNA-based identification if it is exclusively monophyletic in >95% of bootstrap pseudoreplicate phylogenetic analyses. Seven of the nine species meet that criterion. Two species (Lucilia coeruleiviridis and Lucilia mexicana) share COI sequence and cannot be distinguished using our reference database. We conclude that DNA-based identification is likely to be successful for the other seven species.

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Citations

Apr 23, 2016·ZooKeys·Kirstin A WilliamsMartin H Villet
Mar 12, 2016·Parasitology Research·Barbara Karolina ZajacRichard Zehner
Sep 4, 2015·Journal of Medical Entomology·Abeer M SalemChristine J Picard
Jun 13, 2016·Forensic Science International : Synergy·Elena PilliSimonetta Lambiase
Jun 8, 2019·Journal of Medical Entomology·M Denise GemmellaroJessica L Ware
Jun 6, 2020·Journal of Forensic Sciences·Michelle M JonikaAaron M Tarone
Apr 19, 2017·Journal of Medical Entomology·Meenakshi Bharti, Baneshwar Singh
Jul 31, 2018·Journal of Insect Science·Charity G Owings, Christine J Picard
Jan 4, 2017·Mitochondrial DNA. Part B, Resources·Jian ChenXiaohong Li

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