DNA bending in the replication zone of the C3 DNA puff amplicon of Rhynchosciara americana (Diptera: Sciaridae).

Molecular Biology Reports
Adriana FioriniMaria Aparecida Fernandez

Abstract

Intrinsic bent DNA sites were identified in the 4289 bp segment encompassing the replication zone which directs DNA amplification and transcription of the C3-22 gene of Rhynchosciara americana. Restriction fragments showed reduced electrophoretic mobility in polyacrylamide gels. The 2D modeling of the 3D DNA path and the ENDS ratio values obtained from the dinucleotide wedge model of Trifonov revealed the presence of four major bent sites, positioned at nucleotides -6753, -5433, -5133 and -4757. Sequence analysis showed that these bends are composed of 2-6 bp dA.dT tracts in phase with the DNA helical repeat. The circular permutation analysis permitted the verification that the fragments containing the bending sites promote curvature in other sequence contexts. Computer analyses of the 4289 bp sequence revealed low helical stability (DeltaG values), negative roll angles indicating a narrow minor groove and a putative matrix attachment region. The data presented in this paper add to information about the structural features involved in this amplified segment.

References

Nov 14, 1978·Biochemical and Biophysical Research Communications·S M de Toledo, F J Lara
Feb 1, 1992·Molecular Microbiology·M Roberge, S M Gasser
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BolshoyE N Trifonov
Mar 25, 1990·Nucleic Acids Research·T T Eckdahl, J N Anderson
Feb 1, 1989·Journal of Bacteriology·X N DongR H Rownd
Apr 24, 1987·Science·K Zahn, F R Blattner
Jul 1, 1988·Molecular and Cellular Biology·J S WilliamsJ N Anderson
Apr 16, 1987·Nature·L E Ulanovsky, E N Trifonov
Oct 26, 1987·Nucleic Acids Research·T T Eckdahl, J N Anderson
Nov 6, 1986·Nature·M SnyderR W Davis
Apr 10, 1986·Nature·H S KooD M Crothers
May 1, 1993·Journal of Cellular Biochemistry·T Boulikas
Jan 1, 1993·Annual Review of Biochemistry·M L DePamphilis
Jan 1, 1995·International Review of Cytology·T Boulikas
Apr 15, 1996·Genomics·J A KramerS A Krawetz
Jun 15, 1996·Nucleic Acids Research·M Marilley, P Pasero
Feb 10, 1998·BioTechniques·S J Sperbeck, G J Wistow
Nov 3, 1998·Biopolymers·O N de Souza, R L Ornstein
May 12, 2000·Journal of Structural Biology·M L DePamphilis
Oct 3, 2000·The Journal of Biological Chemistry·R C BashD Lohr
Aug 14, 2001·Journal of Cellular Biochemistry·A FioriniM A Fernandez
Apr 1, 1995·Virology·P Suárez-LópezC Gutiérrez
Dec 20, 2002·Journal of Structural Biology·Susan A GerbiJohn M Waggener
Sep 16, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Giuseppe BiamontiArturo Falaschi

❮ Previous
Next ❯

Citations

Feb 17, 2009·Molecular Genetics and Genomics : MGG·Fabrícia GimenesMaria Aparecida Fernandez
Feb 12, 2014·Biochemistry. Biokhimii︠a︡·Q A Lima NetoM A Fernandez
Apr 9, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F GimenesM A Fernandez
Feb 17, 2015·TheScientificWorldJournal·Shelly SehgalM K Dhar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Fabrícia GimenesMaria Aparecida Fernandez
Genetics and Molecular Research : GMR
Fabrícia GimenesMaria Aparecida Fernandez
© 2022 Meta ULC. All rights reserved