DNA bending in transcription initiation

Biochemistry
Vladimir TchernaenkoLeonard C Lutter

Abstract

Electrophoretic mobility shift (bandshift) phasing analysis and rotational variant topological analysis were performed on initiation complexes formed on the bacteriophage lambda PR promoter. Both the open complex and an abortive complex containing a short RNA primer extending to +3 were characterized. The two methods were used to analyze a series of constructs containing tandemly repeated copies of the PR promoter, with the repeat length increased in single base pair increments to progressively change the rotational setting of adjacent copies. The phasing effect observed in bandshift analysis of open complexes formed on this set of constructs provided qualitative evidence for the presence of a bend. Subsequent rotational variant topological analysis confirmed this and quantified the overall bend angle in the open complex as well as in the +3 abortive complex: a bend of 49 degrees +/- 7 degrees was measured for the open complex, while a bend of 47 degrees +/- 11 degrees was measured for the +3 complex, i.e., the two bends are the same. However, the topological results are not consistent with extensive superhelical wrapping of DNA on either complex as has been proposed. The two complexes do differ in the size of the transcription...Continue Reading

References

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Sep 6, 1996·Journal of Molecular Biology·L C LutterC R Calladine
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Aug 17, 1999·The EMBO Journal·C RivettiC Bustamante
Aug 18, 2004·Journal of Molecular Biology·Vladimir TchernaenkoLeonard C Lutter
Feb 1, 2005·FEBS Letters·Hinrich BoegerRoger D Kornberg
May 24, 2005·Journal of Molecular Biology·Kirit R Pindolia, Leonard C Lutter

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Citations

Jul 14, 2010·Functional & Integrative Genomics·Olga V Anatskaya, Alexander E Vinogradov
Apr 28, 2009·Computational Biology and Chemistry·Andrew Shelenkov, Eugene Korotkov
Jan 14, 2009·International Journal of Cardiology·Olga V AnatskayaAlexander E Vinogradov

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