PMID: 2494661Apr 1, 1989Paper

DNA bending is induced by a transcription factor that interacts with the human c-FOS and alpha-actin promoters

Proceedings of the National Academy of Sciences of the United States of America
T A GustafsonL Kedes

Abstract

Conserved sequence elements in the human cardiac and skeletal alpha-actin promoters that contain the CC(A + T-rich)6GG motif have been shown to regulate transcription of these genes. A similar sequence is found in the serum response element of the human c-FOS gene. In this study, we demonstrate that indistinguishable proteins bind to each of five CC(A + T-rich)6GG elements examined in the human cardiac and skeletal alpha-actin promoters and the c-FOS serum response element. Using electrophoretic techniques, we show that these factors induce a stable bend in the DNA upon binding, and the bend center is shown to coincide with the CC(A + T-rich)6GG element. In addition, the ability to bend DNA is retained by a small proteolytic fragment of the protein, suggesting that the DNA-binding domain of the protein is resistant to proteases and is sufficient to bend DNA.

References

Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C Moskaluk, D Bastia
Apr 6, 1987·Nature·J E AndersonS C Harrison
Aug 21, 1986·Nature·J GriffithM Ptashne
Nov 6, 1986·Nature·M SnyderR W Davis
Aug 15, 1985·Nature·J E AndersonS C Harrison
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M BetterH Echols
May 24, 1984·Nature·C A FrederickJ M Rosenberg
Nov 1, 1982·Biopolymers·O J Lumpkin

❮ Previous
Next ❯

Citations

Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P C van der Vliet, C P Verrijzer
Dec 1, 1995·Molecular and Biochemical Parasitology·G C Oliveira, W M Kemp
Jul 1, 1993·Domestic Animal Endocrinology·J R Malayer, J Gorski
Jun 10, 2006·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Sumedha GunewardenaZhaolei Zhang
Nov 11, 1990·Nucleic Acids Research·R Pollock, R Treisman
Dec 25, 1991·Nucleic Acids Research·S L DanilitionN G Miyamoto
Jul 11, 1995·Nucleic Acids Research·A D Sharrocks, P Shore
Apr 11, 1995·Nucleic Acids Research·D L SmithA D Johnson
Jan 12, 1990·European Journal of Biochemistry·H Eisenberg
Mar 12, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J P BidwellJ Hock
Oct 1, 1994·Critical Reviews in Oncology/hematology·M Piechaczyk, J M Blanchard
Apr 30, 2015·PloS One·Josef ŠtěpánekChristian Zentz
Nov 12, 2009·Biochemical and Biophysical Research Communications·Josef StepánekChristian Zentz
Nov 12, 2013·Seminars in Immunology·Rut Valdor, Fernando Macian
Mar 5, 1999·Journal of Molecular Biology·A G West, A D Sharrocks
Aug 10, 1995·Nature·L PellegriniT J Richmond
Mar 15, 1990·International Journal of Cancer. Journal International Du Cancer·A N MalviyaG Vincendon
Mar 17, 1995·The Journal of Biological Chemistry·P ShoreA D Sharrocks
Apr 1, 1995·European Journal of Biochemistry·P Shore, A D Sharrocks
May 1, 1997·Molecular and Cellular Biology·A G WestA D Sharrocks
Dec 1, 1991·Molecular and Cellular Biology·M W Leonard, R K Patient
May 1, 1996·Molecular and Cellular Biology·W D Cress, J R Nevins
May 1, 1992·Molecular and Cellular Biology·A M Nardulli, D J Shapiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.