DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate

Bioconjugate Chemistry
P BigeyPeter E Nielsen

Abstract

A cationic manganese porphyrin-peptide nucleic acid (PNA) conjugate has been prepared and used to cleave a double-stranded DNA target. Cleavage experiments were performed with a 247-base pair restriction DNA fragment containing a 10-base pair homopurine binding target for the PNA. Oxidative activation by this Mn porphyrin-PNA conjugate leads to sequence specific, 3'-staggered cleavage of both DNA strands near the strand displacement junction. Furthermore, the Mn porphyrin-PNA porphyrin conjugates bind over 100-fold better to double-stranded DNA compared to the native PNA.

Citations

May 12, 2000·Biochimica Et Biophysica Acta·H J LarsenP E Nielsen
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·D RamaiahG B Schuster
Nov 13, 2009·Organic & Biomolecular Chemistry·Anna M SosniakNils Metzler-Nolte
May 5, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Gilles GasserNils Metzler-Nolte
Jul 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lei ShiHai-Yang Liu
May 12, 2011·Chemistry & Biodiversity·Yu ZhangXiao-Qi Yu
Jan 27, 2010·Chemical Reviews·Marguerite Pitié, Geneviève Pratviel
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