DNA binding and recognition by the IIs restriction endonuclease MboII

The Journal of Biological Chemistry
Meera SoundararajanBernard A Connolly

Abstract

The type IIs restriction endonuclease MboII recognizes nonsymmetrical GAAGA sites, cutting 8 (top strand) and 7 (bottom strand) bases to the right. Gel retardation showed that MboII bound specifically to GAAGA sequences, producing two distinct complexes each containing one MboII and one DNA molecule. Interference analysis indicated that the initial species formed, named complex 1, comprised an interaction between the enzyme and the GAAGA target. Complex 2 involved interaction of the protein with both the GAAGA and the cutting sites. Only in the presence of divalent metal ions such as Ca(2+) is the conversion of complex 1 to 2 rapid. Additionally, a very retarded complex was seen with Ca(2+), possibly a (MboII)(2)-(DNA)(2) complex. Plasmids containing a single GAAGA site were hydrolyzed slowly by MboII. Plasmids containing two sites were cut far more rapidly, suggesting that the enzyme requires two recognition sites in the same DNA molecule for efficient hydrolysis. MboII appears to have a mechanism similar to the best characterized type IIs enzyme, FokI. Both enzymes initially bind DNA as monomers, followed by dimerization to give an (enzyme)(2)-(DNA)(2) complex. Dimerization is efficient only when the two target sites are loca...Continue Reading

References

Feb 11, 1992·Nucleic Acids Research·M SektasA J Podhajska
Mar 11, 1991·Nucleic Acids Research·H BocklageB Müller-Hill
May 5, 1991·Journal of Molecular Biology·F W Studier
Apr 1, 1991·Gene·W SzybalskiA J Podhajska
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1990·Methods in Enzymology·C M Stoscheck
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W R McClure
Jun 15, 1980·Journal of Molecular Biology·N L BrownM Smith
May 19, 1995·Gene·M SektasA J Podhajska
Jan 1, 1994·Methods in Molecular Biology·S E HalfordI B Vipond
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D R LesserL Jen-Jacobson
May 15, 1997·European Journal of Biochemistry·A Pingoud, A Jeltsch
Jul 3, 1997·Nature·D A WahA K Aggarwal
Nov 5, 1997·The Journal of Biological Chemistry·H G NastriP D Riggs
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·D A WahA K Aggarwal
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BitinaiteI Schildkraut
Jun 17, 1999·Journal of Molecular Biology·M L EmbletonS E Halford
Oct 6, 1999·Current Opinion in Chemical Biology·R A Kovall, B W Matthews
Oct 16, 1999·Journal of Molecular Biology·V SiksnysR Huber
Dec 14, 1999·The Journal of Biological Chemistry·D T BilcockS E Halford
Mar 4, 2000·The Journal of Biological Chemistry·N A GormleyS E Halford
Jan 11, 2000·Nucleic Acids Research·R J Roberts, D Macelis
Mar 20, 2001·Journal of Molecular Biology·L E EnglerL Jen-Jacobson
Aug 9, 2001·Journal of Molecular Biology·E S VanameeA K Aggarwal
Dec 1, 2001·The Journal of Biological Chemistry·Abigail J BathStephen E Halford

❮ Previous
Next ❯

Citations

Jun 8, 2002·Journal of Molecular Biology·Shelley A Williams, Stephen E Halford
Mar 14, 2003·Journal of Molecular Biology·Lucy E DanielsStephen E Halford
Jul 1, 2004·Nucleic Acids Research·Darren M GowersStephen E Halford
Mar 25, 2006·Nucleic Acids Research·Lucy E CattoStephen E Halford
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Giedrius SasnauskasVirginijus Siksnys
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gregory J GemmenDouglas E Smith
Jul 27, 2005·Biochemical and Biophysical Research Communications·Mala ManiSrinivasan Chandrasegaran
Apr 14, 2005·Journal of Molecular Biology·Daniel F HeiterGeoffrey G Wilson
Mar 25, 2011·Biochemical Society Transactions·Stephen E HalfordKelly L Sanders
Feb 3, 2007·Journal of Molecular Biology·Jacqueline J T MarshallStephen E Halford
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Stephen E HalfordMark D Szczelkun
Jan 28, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mélanie J ChagnonMichel L Tremblay
Dec 1, 2001·The Journal of Biological Chemistry·Abigail J BathStephen E Halford
Jul 23, 2008·Biochemistry·Arturas JakubauskasArvydas Janulaitis

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.