DNA binding, cleavage, and cytotoxic activity of the preorganized dinuclear zinc(II) complex of triazacyclononane derivatives

Bioconjugate Chemistry
Xin ShengQiang Xu

Abstract

A preorganized cleft dinuclear zinc(II) complex of 2,6-bis(1-methyl-1,4,7-triazacyclonon-1-yl)pyridine 1 as an artificial nuclease was prepared via an improved method. The interactions of 1, 2 [1,4,7-triazacyclononane (TACN)], and their zinc(II) complexes with calf thymus DNA were studied by spectroscopic techniques, including fluorescence and CD spectroscopy. The results indicate that the DNA binding affinities of these compounds are in the following order: Zn(II)2 -1 > Zn(II) -2 > 1 > 2. The binding constants of the Zn (II)2 -1 and Zn(II)-2 complexes are 3.57 x 10(6) and 1.43 x 10(5) M(-1), respectively. Agarose gel electrophoresis was used to assess the plasmid pUC 19 DNA cleavage activities in the presence of the dinuclear Zn (II)2 -1 complex, which exhibits powerful DNA cleavage efficiency. Kinetic data for DNA cleavage promoted by the Zn(II)2 -1 complex under physiological conditions give the observed rate constant ( k obs) of 0.136 h(-1), which shows an 10(7)-fold rate acceleration over uncatalyzed supercoiled DNA. The comparison of the dinuclear Zn(II)2 -1 complex with the mononuclear zinc(II) complex of 1,4,7-triazacyclononane indicates that the DNA cleavage acceleration promoted by the Zn(II)2 -1 complex is due to the...Continue Reading

References

Mar 20, 1991·Journal of Molecular Biology·E E Kim, H W Wyckoff
Mar 6, 1987·Science·F H Westheimer
Aug 1, 1989·British Journal of Cancer·J M Sargent, C G Taylor
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T A Steitz, J A Steitz
Jan 7, 1993·Nature·J A PiccirilliT R Cech
Jul 18, 2001·Journal of the American Chemical Society·M E BranumL Que
Jul 18, 2001·Journal of the American Chemical Society·C SissiP Scrimin
Dec 12, 2001·Current Opinion in Chemical Biology·J A Cowan
Dec 26, 2001·Chemical Reviews·K E ErkkilaJ K Barton
Feb 19, 2002·Chemical Reviews·Wendy Knapp Pogozelski, Thomas D. Tullius
Feb 19, 2002·Chemical Reviews·Richard M. Burger
Feb 19, 2002·Chemical Reviews·Bruce Armitage
Feb 19, 2002·Chemical Reviews·David R. McMillin, Kristina M. McNett
Feb 19, 2002·Chemical Reviews·Scott R. Rajski, Robert M. Williams
Jul 18, 2002·Journal of Inorganic Biochemistry·Jie LiuLiangnian Ji
Dec 12, 2002·Journal of the American Chemical Society·Tim HumphryAlvan C Hengge
Jan 16, 2003·Bioconjugate Chemistry·Jakub Fichna, Anna Janecka
Jul 11, 2003·Bioorganic & Medicinal Chemistry Letters·Feng LiangMengxiang Sun
Aug 28, 2003·Current Opinion in Chemical Biology·Christiana Xin Zhang, Stephen J Lippard
Dec 4, 2003·Chemical Communications : Chem Comm·Meng-Yin YangJanet R Morrow
Feb 12, 2004·Chemical Reviews·Shin Aoki, Eiichi Kimura
Apr 6, 2004·Current Opinion in Chemical Biology·Janet R Morrow, Olga Iranzo
Apr 9, 2004·Journal of the American Chemical Society·Elisa BoseggiaGiuseppe Zagotto
Sep 24, 2004·Dalton Transactions : an International Journal of Inorganic Chemistry·Tarkeshwar GuptaAkhil R Chakravarty
Oct 29, 2004·Journal of Medicinal Chemistry·Laurie L ParkerIan J Stratford
Nov 18, 2004·Bioconjugate Chemistry·Todd M CorneillieClaude F Meares
Nov 26, 2004·Journal of the American Chemical Society·Akiko Nomura, Yukio Sugiura
Feb 11, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Konstantina KaridiJan Reedijk
Mar 29, 2005·Inorganic Chemistry·Claudia SissiUmberto Tonellato
Jun 9, 2005·Journal of the American Chemical Society·Yan Jin, J A Cowan
Sep 30, 2005·Journal of the American Chemical Society·Guoqiang FengNicholas H Williams
Mar 7, 2006·Dalton Transactions : an International Journal of Inorganic Chemistry·Carla BazzicalupiBarbara Valtancoli

❮ Previous
Next ❯

Citations

Oct 26, 2011·Future Medicinal Chemistry·Béla Gyurcsik, Anikó Czene
Apr 26, 2014·European Journal of Medicinal Chemistry·Natarajan Raman, Narayanaperumal Pravin
Nov 16, 2010·Bioinorganic Chemistry and Applications·Konstantis F KonidarisEvy Manessi-Zoupa
Jun 26, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jun TanBochu Wang
Sep 8, 2012·Bioinorganic Chemistry and Applications·Nahid Shahabadi, Somaye Mohammadi
Feb 26, 2016·Critical Reviews in Analytical Chemistry·Bin YangLianming Zhang
Jun 12, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ria SanyalDebasis Das
Sep 3, 2014·Metallomics : Integrated Biometal Science·Anikó CzeneBéla Gyurcsik
Apr 15, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Paola F LiguoriDaniela Pucci
May 1, 2012·Chemical Communications : Chem Comm·Fabrizio MancinPaolo Tecilla
Sep 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Sellamuthu AnbuMuthusamy Kandaswamy
Sep 26, 2012·Organic & Biomolecular Chemistry·Li WeiGuo-Yuan Lu
Dec 4, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Ming LiuJing-Yuan Xu
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiahe LiZhen Ma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.